›› 2012, Vol. 55 ›› Issue (1): 24-28.doi:

• 研究论文 • 上一篇    下一篇

即时浸酸显著提高滞育性家蚕卵辅酶Ⅰ和Ⅱ含量

姚金美, 万华星, 司马杨虎, 赵林川   

  1. 苏州大学基础医学与生物科学学院, 江苏苏州 215123
  • 收稿日期:2011-10-09 修回日期:2011-12-23 出版日期:2012-01-20 发布日期:2012-01-20
  • 通讯作者: 赵林川 E-mail:sdzlc2008@163.com
  • 作者简介: 姚金美, 女, 1986年8月生, 江西上饶人, 硕士研究生, 主要从事自由基生物学研究, E-mail: yaojm3948@163.com
  • 基金资助:

    国家自然科学基金项目(31072090); 现代农业产业技术体系(CARS-22-ZJ0104)

Instant hydrochloride acid soaking enhances NAD(H) and NADP(H) levels in diapausing eggs of the silkworm, Bombyx mori

YAO Jin-Mei, WAN Hua-Xing, SIMa Yang-Hu, ZHAO Lin-Chuan   

  1. College of Preclinical Medicine and Biological Science, Soochow University, Suzhou, Jiangsu 215123, China
  • Received:2011-10-09 Revised:2011-12-23 Online:2012-01-20 Published:2012-01-20
  • Contact: ZHAO Lin-Chuan E-mail:sdzlc2008@163.com
  • About author:yaojm3948@163.com

摘要: 即时浸酸在阻止家蚕Bombyx mori卵滞育发动的同时, 提高了其呼吸耗氧量, 抑制了山梨醇积累。本研究利用HPLC法测定了家蚕滞育卵和5 min即时浸酸滞育性卵中辅酶Ⅰ和Ⅱ含量。结果表明: 产下后24-72 h, 家蚕滞育卵中NAD, NADH, NADP和NADPH含量分别下降了30%, 37%, 50%和4%; 而即时浸酸滞育性卵中分别增加了77%, 46%, 142%和241%。不过, 即时浸酸并未显著改变滞育性家蚕卵中NADH/NAD和NADPH/NADP比值。据此推测, 即时浸酸提高滞育性家蚕卵辅酶Ⅰ含量与其呼吸耗氧量增加有关; 即时浸酸显著提高辅酶Ⅱ含量与山梨醇积累抑制无关, 而主要与生物合成加强有关。

关键词: 家蚕, 滞育, 滞育卵, 即时浸酸, 辅酶Ⅰ, 辅酶Ⅱ

Abstract: For diapausing eggs of the silkworm, Bombyx mori, diapause initiation is prevented with instant hydrochloric acid (HCl) soaking, which can cause the marked enhancement of oxygen consumption and the inhibition of sorbitol accumulation. NAD(H) and NADP(H) levels in diapause eggs and those soaked with HCl for 5 min at 24 h after oviposition were determined using HPLC methods. From 24 h to 72 h after oviposition, levels of NAD, NADH, NADP and NADPH in diapausing eggs decreased by 30%, 37%, 50% and 4%, respectively, whereas those in diapausing eggs soaked with HCl increased by 77%, 46%, 142% and 241%, respectively. Moreover, no significant effect of HCl on both NADH/NAD and NADPH/NADP ratios was observed. It is so inferred that, in diapausing eggs soaked with HCl, the marked increase of NAD(H) is involved in the marked enhancement of oxygen consumption, whereas the enhancement of NADP(H) is related to the stronger biosynthesis rather than the inhibition of sorbitol accumulation.

Key words: Bombyx mori, diapause, diapause eggs, instant HCl soaking, NAD(H), NADP(H)

中图分类号: 

  • Q966