虫酰肼对甜菜夜蛾多巴脱羧酶和酪氨酸羟化酶的抑制作用

›› 2012, Vol. 55 ›› Issue (1): 29-35.doi:

虫酰肼对甜菜夜蛾多巴脱羧酶和酪氨酸羟化酶的抑制作用

王涛, 邱秀翠, 焦艳艳, 刘辉, 刘永杰

山东农业大学植物保护学院，山东泰安 271018

收稿日期:2011-09-02 修回日期:2011-12-23 出版日期:2012-01-20 发布日期:2012-01-20
通讯作者: 刘永杰 E-mail:lyj@sdau.edu.cn
作者简介:王涛，男， 1986年2月生，硕士研究生，研究方向为昆虫毒理学， E-mail: wangtao226@163.com
基金资助:
山东省“十一五”科技攻关项目（2008GG10009032）；山东省现代农业产业技术体系蔬菜专项

Inhibitory effects of tebufenozide on dopa decarboxylase and tyrosine hydroxylase in 5th instar larvae of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)

WANG Tao, QIU Xiu-Cui, JIAO Yan-Yan, LIU Hui, LIU Yong-Jie

College of Plant Protection, Shandong Agricultural University, Tai’an, Shandong 271018, China

Received:2011-09-02 Revised:2011-12-23 Online:2012-01-20 Published:2012-01-20
Contact: LIU Yong-Jie E-mail:lyj@sdau.edu.cn
About author:wangtao226@163.com

摘要/Abstract

摘要： 虫酰肼模拟昆虫蜕皮激素的作用干扰新表皮的形成。为了探讨虫酰肼对昆虫新表皮形成的影响是否与抑制表皮形成相关酶的活性有关，本研究应用高效液相色谱-荧光检测法（HPLC-RP），测定了甜菜夜蛾Spodoptera exigua 5龄幼虫用虫酰肼处理不同时间（24， 48和72 h）后多巴脱羧酶和酪氨酸羟化酶的活性。结果表明：用LC₁₁（28.41 μmol/L）和LC₃₃（85.23 μmol/L）两个亚致死剂量的虫酰肼处理5龄幼虫后，多巴脱羧酶和酪氨酸羟化酶的活性均受到明显抑制，高浓度的抑制作用大于低浓度的抑制作用。随着处理时间的延长，同一剂量的抑制作用逐渐增强。进一步测定虫酰肼处理24， 48和72 h后5龄幼虫血淋巴、脂肪体、中肠、表皮和头部的多巴脱羧酶和酪氨酸羟化酶的活性，可看出虫酰肼对幼虫不同组织的多巴脱羧酶和酪氨酸羟化酶的活性也具有相似的抑制作用。结果提示，虫酰肼对甜菜夜蛾幼虫多巴脱羧酶和酪氨酸羟化酶活性具有明显抑制作用，幼虫新表皮形成受阻可能与虫酰肼抑制多巴脱羧酶和酪氨酸羟化酶的活性有关。

关键词: 甜菜夜蛾, 虫酰肼, 多巴脱羧酶, 酪氨酸羟化酶, 酶活性, 抑制效应

Abstract: Tebufenozide acting in a similar fashion of ecdysone was found to produce phenotypic effects that finally interfere the formation of new larval cuticle in insects. To explore the effects of tebufenozide on the enzyme activities related to the formation of new cuticle in insects, high performance liquid chromatography (HPLC)-fluorescent assay was used to determine the activities of dopa decarboxylase (DDC) and tyrosine hydroxylase (TH) in 5th instar larvae of Spodoptera exigua which were continuously treated with tebufenozide for different time. The results indicated that after the larvae were treated with two sublethal concentrations (LC₁₁28.41 μmol/L and LC₃₃ 85.23 μmol/L) of tebufenozide, the activities of DDC and TH were significantly inhibited, and the inhibitory effect was more marked when the larvae were treated with tebufenozide at the high sublethal concentration than at the low sublethal concentration. The inhibition rates of DDC and TH activities were raised with treatment time at the same sublethal concentration. Tebufenozide had the similar inhibitory effects on DDC and TH activities in the midgut, head, fat body, hemolymph and cuticle of the 5th instar larvae as on those in the whole larval body. It is so concluded that the interference in the formation of new larval cuticle is likely related to the inhibition of DDC and TH activities by tebufenozide.

Key words: Spodoptera exigua, tebufenozide, dopa decarboxylase (DDC), tyrosine hydroxylase (TH), enzyme activity, inhibitory effect

中图分类号:

Q965.9

王涛, 邱秀翠, 焦艳艳, 刘辉, 刘永杰. 虫酰肼对甜菜夜蛾多巴脱羧酶和酪氨酸羟化酶的抑制作用[J]. , 2012, 55(1): 29-35.

WANG Tao, QIU Xiu-Cui, JIAO Yan-Yan, LIU Hui, LIU Yong-Jie. Inhibitory effects of tebufenozide on dopa decarboxylase and tyrosine hydroxylase in 5th instar larvae of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)[J]. , 2012, 55(1): 29-35.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/

http://www.insect.org.cn/CN/Y2012/V55/I1/29

[1]	陈菊红, 崔娟, 张金平, 毕锐, 高宇, 徐伟, 史树森. 温度胁迫对点蜂缘蝽成虫呼吸代谢关键酶活性的影响[J]. , 2018, 61(9): 1003-1009.
[2]	武海斌, 宫庆涛, 陈珍珍, 姜莉莉, 公义, 许永玉, 孙瑞红. 昆虫病原线虫和噻虫嗪混用对韭蛆的杀虫效果及其体内保护酶和解毒酶活性的影响[J]. , 2018, 61(7): 851-859.
[3]	岳文波, 郅军锐, 刘利, 叶茂, 张向前, 曾广 . 害虫取食和机械损伤对菜豆不同部位叶片防御酶活性的影响[J]. , 2018, 61(7): 860-870.
[4]	邹金城, 孙宇, 杨勇, 杨益众, 张子昕, 王思雨, 苏宏华. 镉胁迫显著影响甜菜夜蛾成虫的飞行能力[J]. , 2017, 60(9): 1021-1030.
[5]	钱雪, 王月莹, 谢欢欢, 窦洁, 李占武, Roman JASHENKO, 季荣. 温度对西伯利亚蝗呼吸代谢关键酶活性的影响[J]. , 2017, 60(5): 499-504.
[6]	姜丽娜, 钱蕾, 喜超, 王雪丽, 杨璐, 桂富荣. CO₂浓度升高对不同寄主植物上西花蓟马和花蓟马成虫体内消化酶活性的影响[J]. , 2017, 60(3): 237-246.
[7]	谭永安, 赵旭东, 郝德君, 肖留斌, 柏立新, 赵静, 孙洋, 姜义平. 绿盲蝽磷脂酶C(AlPLC)的表达、纯化及酶学性质[J]. , 2017, 60(11): 1247-1254.
[8]	任小双, 张兰, 张燕宁, 毛连纲, 蒋红云. 喜树碱诱导甜菜夜蛾细胞凋亡线粒体途径的MPTP依赖性[J]. , 2017, 60(10): 1105-1113.
[9]	郅军锐, 田甜, 温娟, 刘勇. 西花蓟马或二斑叶螨为害的菜豆对两者间后取食者体内保护酶和解毒酶活性的影响[J]. , 2016, 59(7): 707-715.
[10]	付盈盈, 马玉堃, 任淑文, 杨立军, 刘春光,付海燕, 侯跃莹, 杨峰山. 革兰氏阳性菌苏云金芽孢杆菌和革兰氏阴性菌大肠杆菌诱导的柞蚕蛹生理指标变化[J]. , 2016, 59(2): 192-199.
[11]	赵爱平, 孙聪, 展恩玲, 仵均祥, 李怡萍. 蛋白酶抑制剂对梨小食心虫幼虫中肠蛋白酶活性的影响[J]. , 2016, 59(10): 1069-1078.
[12]	张佩, 张兰, 张燕宁, 贾伟, 蒋红云. 甜菜夜蛾拓扑异构酶I氨基酸突变对其DNA解旋活性的影响[J]. , 2015, 58(9): 933-940.
[13]	赵吕权, 吴红军, 朱道弘. 丽斗蟋翅二型雄虫食物消化能力及消化酶活性比较[J]. , 2015, 58(7): 731-738.
[14]	余慧灵, 向兴, 袁贵鑫, 陈羿渠, 王学贵. 溴氰虫酰胺亚致死剂量对甜菜夜蛾生长发育及体内解毒酶活性的影响[J]. , 2015, 58(6): 634-641.
[15]	袁瑞玲, 王晓渭, 杨珊, 陈鹏. 不同日龄和吊飞过程中桔小实蝇成虫飞行肌能量代谢相关酶活性的变化[J]. , 2015, 58(5): 471-478.