›› 2012, Vol. 55 ›› Issue (1): 46-54.doi:

• 研究论文 • 上一篇    下一篇

蜀柏毒蛾生殖行为及性信息素产生与释放节律

张坤胜, 杨伟, 卓志航, 邓忠彬, 杨春平, 杨桦, 周建华, 肖银波, 贾玉珍   

  1. 四川农业大学林学院, 四川省林业生态工程省级重点实验室, 四川雅安 625014
  • 收稿日期:2011-07-29 修回日期:2011-11-21 出版日期:2012-01-20 发布日期:2012-01-20
  • 通讯作者: 杨伟 E-mail:ywei0218@yahoo.com.cn
  • 作者简介:张坤胜, 男, 1986年生, 广西防城港人, 硕士研究生, 从事森林病虫害防治, E-mail: zhangkunsheng@126.com
  • 基金资助:

    四川农业大学“211工程”双支计划资助项目(00370101); 四川农业大学长江上游生态林业工程建设资助项目; 林业公益性行业科研专项(2010040036)

Reproduction behavior and circadian rhythm of sex pheromone production and release in Parocneria orienta (Lepidoptera: Lymantriidae)

ZHANG Kun-Sheng, YANG Wei, ZHUO Zhi-Hang, DENG Zhong-Bin, YANG Chun-Ping, YANG Hua, ZHOU Jian-Hua, XIAO Yin-Bo, JIA Yu-Zhen   

  1. Key Laboratory of Ecological Forestry Engineering of Sichuan Province, College of Forestry, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
  • Received:2011-07-29 Revised:2011-11-21 Online:2012-01-20 Published:2012-01-20
  • Contact: YANG Wei E-mail:ywei0218@yahoo.com.cn
  • About author:zhangkunsheng@126.com

摘要: 为了探索蜀柏毒蛾Parocneria orienta Chao性信息素产生和释放规律, 为利用性信息素监测和防治蜀柏毒蛾奠定基础, 本研究在野外及室内温度22±1℃、 相对湿度75%~80%、 光周期14L∶10D条件下观察研究了蜀柏毒蛾成虫的羽化、 求偶、 交尾、 产卵行为, 触角电位反应测定处女雌蛾性信息素产生与释放的时辰节律。结果表明: 蜀柏毒蛾羽化行为全天可见, 主要集中在1:00-5:00, 占总羽化量的44.94%, 7:30-11:00进行婚飞和交尾, 交尾高峰期出现在8:30左右, 交配时间少则2 h, 多则8 h, 求偶、 交配均发生在光期。随着日龄的增加, 召唤时间前移并且延长, 1日龄的处女雌蛾交尾时间较短; 雌蛾羽化当天就可交尾, 2日龄雌蛾交尾率最高, 达36.67%。雌蛾分多处产卵, 雌蛾一生最高产卵量达402粒, 最低产卵量为78粒。羽化当天的雌蛾体内性信息素含量较低, 第2天最高, 以后逐日下降; 2日龄蜀柏毒蛾处女雌蛾性信息素的产生量从7:00起逐渐增加, 8:30-9:30时最高, 9:30后逐渐减小。雄蛾对处女雌蛾腺体提取物的触角电位反应在8:30-9:00最强, 说明8:30-9:00是雌蛾产生和释放性信息素的高峰期。蜀柏毒蛾的羽化、 求偶、 交尾及性信息素的产生与释放存在一定的时辰节律, 野外处女雌蛾诱蛾试验证实了性信息素释放与交配行为在时辰节律上的一致性。

关键词: 蜀柏毒蛾, 生殖行为, 羽化, 交尾, 性信息素, 时辰节律

Abstract: In order to explore the rhythm of sex production and release of Parocneria orienta Chao, to establish the basis for using sex pheromone to monitor and control the pest, the emergence, calling, mating and oviposition behaviors of P. orienta were studied through observations in the fields and in the laboratory under the conditions of 22±1℃, 75%-80% RH and 14L∶10D photoperiod. The circadian rhythm of sex pheromone production and release in P. orienta were studied by determining the electroantennogram (EAG) response. The results showed that the emergence of P. orienta happened during the day, especially from 1:00 to 5:00, accounting for 44.94% of the total. The nuptial flight and mating behaviors took place from 7:30 to 11:00. The peak of mating occurred at 8:30 or so. The calling and mating behaviors were both observed during the photophase. Calling time varied with the age of female, with older (2- to 3-dayold) virgin females calling later and longer calling duration than younger (1-day-old) females. The 1-day-old female had shorter lasting time of mating. The mating rate of 2-day-old females was the highest (36.67%). The maximum oviposition in the whole life of a female was up to 402 eggs, and the minimum was 78 eggs. The amount of pheromone in the gland was relatively low at 1 d after emergence, with a peak at 2 d after emergence, and then decreased gradually day by day. The amount of pheromone of 2-day-old virgin females began to increase gradually post 7:00, with the peak between 8:30 and 9:30, and then decreased gradually post 9:30. EAG response was the strongest from 8:30 to 9:00. The peak of sex pheromone production and release of P. orienta occurred between 8:30 and 9:00. There was a circadian rhythm in the emergence, calling and mating, and the sex production and release of adults. The sex pheromone release and mating behavior of P. orienta were synchronized, which was confirmed in field tests by using virgin females to trap males.

Key words: Parocneria orienta, reproductive behavior, emergence, mating, sex pheromone, circadian rhythm

中图分类号: 

  • Q968