›› 2012, Vol. 55 ›› Issue (8): 933-940.doi:

• 研究论文 • 上一篇    下一篇

抗生素处理对感染Wolbachia的丽蚜小蜂生殖的影响

童蕾蕾, 亓兰达, 张帆, 李元喜   

  • 收稿日期:2012-03-25 修回日期:2012-06-25 出版日期:2012-09-20 发布日期:2012-09-20
  • 通讯作者: 李元喜 E-mail:yxli@njau.edu.cn
  • 作者简介:童蕾蕾, 女, 1985年7月生, 硕士研究生, 主要从事昆虫分子生态学研究, E-mail: 2009102099@njau.edu.cn

Effects of antibiotic treatment on reproduction of Encarsia formosa (Hymenoptera: Aphelinidae) infected with Wolbachia

TONG Lei-Lei, QI Lan-Da, ZHANG Fan, LI Yuan-Xi   

  • Received:2012-03-25 Revised:2012-06-25 Online:2012-09-20 Published:2012-09-20
  • Contact: LI Yuan-Xi E-mail:yxli@njau.edu.cn
  • About author:2009102099@njau.edu.cn

摘要: Wolbachia是一类广泛存在于节肢动物体内, 可以对寄主的生殖力及生殖行为产生影响的共生菌。用抗生素可以有效除去寄主体内的Wolbachia。本实验通过喂食浓度分别为1, 5和10 mg盐酸四环素/mL蔗糖水, 结合PCR检测丽蚜小蜂Encarsia formosa体内Wolbachia的去除效果; 解剖观察丽蚜小蜂F0代及F1, F2和F3代怀卵量和卵巢管数量, 评价Wolbachia对丽蚜小蜂生殖的影响。结果显示: 抗生素处理去除Wolbachia后F0代蜂的卵巢管数量与未处理蜂之间无显著差异(P=0.12), 但F1, F2和F3代蜂的卵巢管均为6条, 显著少于F0代蜂的卵巢管数量(P<0.001)。抗生素处理去除Wolbachia后的F0代蜂怀卵量与未处理蜂相比显著下降, 但显著高于经抗生素处理去除Wolbachia后的F1, F2和F3代蜂怀卵量(P<0.001), 后代(F1, F2, F3)蜂之间怀卵量无显著差异(P=0.59)。去除Wolbachia后, 丽蚜小蜂可以产生雄性后代, 但未见交配行为, 且雌蜂可不经交配而产生雌性后代。结果说明, Wolbachia不仅直接影响丽蚜小蜂的怀卵量, 而且还可以通过影响丽蚜小蜂卵巢管的发育影响丽蚜小蜂的怀卵量; 然而, 去除Wolbachia不改变雌蜂的孤雌生殖方式。

关键词: Wolbachia; 丽蚜小蜂; 生殖; 怀卵量; 卵巢管, wsp基因

Abstract: Wolbachia is widely distributed in arthropods and can manipulate the reproduction and fertility of its hosts. Antibiotics can effectively remove Wolbachia from host body. In this study, Wolbachia in Encarsia formosa was removed by feeding wasps with solution of 1, 5 and 10 mg tetracycline hydrochloride/mL sucrose water, and PCR technique was used to detect the completely removing of Wolbachia from female wasps. The numbers of eggs loaded and ovarian tubules of the females were investigated to evaluate the effect of Wolbachia on reproduction of E. formosa. The results indicated that there was no significant difference in the number of ovarian tubules among F0 female of different treatments (P=0.12), whereas there were 6 ovarian tubules in female wasps of Wolbachia free F1, F2 and F3 generations, significantly less than in female wasps of F0 generation (P<0.001). Egg load in F0 generation wasp from which Wolbachia was removed by feeding antibiotics was less than that in the untreated female of F0, but was more than that in F1, F2 or F3 generation (P<0.001). No significant difference in egg load among F1, F2 and F3 generations was found (P=0.59). E. formosa could produce male offsprings after Wolbachia was removed, but no mating behavior was found and females could produce female offsprings without mating. These results suggest that Wolbachia not only affects directly egg load of host but also affects the development of ovarian of host; however, removing of Wolbachia does not change the parthenogenetic reproductive mode of E. formosa.

Key words: Wolbachia, Encarsia formosa, reproduction, egg load, ovarian tubules, wsp gene