转cry1Ab/cry1Ac基因水稻对大螟幼虫体内三种保护酶活性的影响

›› 2012, Vol. 55 ›› Issue (8): 958-963.doi:

转cry1Ab/cry1Ac基因水稻对大螟幼虫体内三种保护酶活性的影响

郭文娟, 陆驰宇, 熊应强, 马鹤娟, 李绍勤

收稿日期:2012-03-02 修回日期:2012-08-02 出版日期:2012-09-20 发布日期:2012-09-20
通讯作者: 李绍勤 E-mail: lishaoqin@mail.hzau.edu.cn
作者简介:郭文娟，女， 1986年生，湖北鄂州人，硕士研究生，研究方向为生物安全评价， E-mail: guowenjuan7788521@163.com

Effects of transgenic cry1Ab/cry1Ac rice on the activities of three protective enzymes in larvae of Sesamia inferens (Lepidoptera: Noctuidae)

GUO Wen-Juan, LU Chi-Yu, XIONG Ying-Qiang, MA He-Juan, LI Shao-Qin

Received:2012-03-02 Revised:2012-08-02 Online:2012-09-20 Published:2012-09-20
Contact: LI Shao-Qin E-mail: lishaoqin@mail.hzau.edu.cn
About author:guowenjuan7788521@163.com

摘要/Abstract

摘要： 为阐明转cry1Ab/cry1Ac基因水稻对大螟Sesamia inferens (Walker)作用的生理生化机制，本研究用转cry1Ab/cry1Ac基因水稻茎秆饲喂大螟3龄和5龄幼虫，采用酶活性测定方法研究了取食转Bt水稻对大螟幼虫体内3种保护酶SOD(superoxide dismutase)、 CAT(catalase)和POD(peroxidase)活性的影响。结果表明，大螟3龄幼虫在取食转基因水稻24 h后SOD活性与对照相比提高了43.44%， 48 h后降至最低值；在取食24 h后POD值达到最高值，其酶活性比对照升高了29.22%，最终在取食48 h后降至最低值，并显著低于对照；在取食转基因水稻4 h后， CAT活性升高了30.33%，在取食48 h后，与对照相比， CAT活性降低了27.01%； 5龄幼虫取食4 h后SOD活性显著高于对照水平， 36 h后降至最低值，与对照相比，活性下降了31.62%；在取食8 h后POD活性达到最高值，与对照相比，升高了73.20%， 36 h后酶活性降至最低值；在取食之初4 h CAT活性达到最高值，与对照相比，其值升高了75.73%，在取食48 h后，其活性与对照相比减少了7.55%。3龄幼虫与5龄幼虫相比，对Bt的抗性水平较低，自身防卫能力较差。结果说明，在取食初期，试虫体内保护酶活性升高，以抵御Bt毒蛋白对虫体的伤害作用，随着取食时间的延长，保护酶活性迅速降低，从而干扰虫体正常的代谢过程，导致虫体出现中毒症状，致使昆虫死亡。

关键词: 大螟, 转基因水稻, 保护酶, SOD, CAT, POD

Abstract: To clarify the physiological and biochemical mechanism of the effect of transgenic cry1Ab/cry1Ac rice on larvae of Sesamia inferens (Walker), the effects of feeding transgenic Bt rice on the activities of three protective enzymes (SOD, CAT and POD) in S. inferens larvae were studied by assaying enzyme activities in the 3rd and 5th instar larvae which were fed with stems of transgenic and non-transgenic rice (control) for different time. The results showed that SOD activity in the 3rd instar larvae fed with Bt rice for 24 h was significantly higher than that of the control (increased by 43.44%), but declined to the minimum at 48 h after feeding. The POD activity in the 3rd larvae fed with Bt rice reached the maximum at 24 h after feeding, and significantly higher than that of the control （increased by 29.22%）, but declined to the minimum at 48 h after feeding which was significantly lower than that of the control. The CAT activity in the 3rd larvae fed with Bt rice for 4 h increased by 30.33%, and decreased by 27.01% at 48 h after feeding compared with that of the control. The SOD activity in the 5th instar larvae was significantly higher than that of the control after the larvae was fed on transgenic rice for 4 h, but declined to the minimum at 36 h after feeding (decreased by 31.62% compared with that of the control). The POD activity reached the maximum and increased by 73.20% compared with that of the control after the larvae was fed on Bt rice for 8 h, but declined to the minimum at 36 h after feeding. The CAT activity reached the maximum and increased by 75.73% after the larvae was fed with transgenic rice for 4 h and decreased by 7.55% at 48 h after feeding compared with that of the control. The resistance level of the 3rd instar larvae was lower than the 5th instar larvae when treated by transgenic Bt rice for the reason that the 3rd instar larvae had lower defense capability. The results suggest that in the initial stage of feeding, the enzyme activity in larvae increases in order to protect larvae from being damaged by Bt toxalbumin. The enzyme activities in larvae rapidly descend and the metabolism of larvae is disturbed due to feeding transgenic rice with extension of feeding time, causing the poisoning symptoms and even death of larvae.

Key words: Sesamia inferens, transgenic rice, protective enzymes, SOD, CAT, POD

郭文娟, 陆驰宇, 熊应强, 马鹤娟, 李绍勤. 转cry1Ab/cry1Ac基因水稻对大螟幼虫体内三种保护酶活性的影响[J]. , 2012, 55(8): 958-963.

GUO Wen-Juan, LU Chi-Yu, XIONG Ying-Qiang, MA He-Juan, LI Shao-Qin. Effects of transgenic cry1Ab/cry1Ac rice on the activities of three protective enzymes in larvae of Sesamia inferens (Lepidoptera: Noctuidae)[J]. , 2012, 55(8): 958-963.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/

http://www.insect.org.cn/CN/Y2012/V55/I8/958

[1]	苟长青, 孙鹏, 刘端春, 迪丽努尔·艾麦提, 冯宏祖. 牧草盲蝽危害对寄主植物体内营养物质含量及保护酶活性的影响[J]. , 2018, 61(8): 976-983.
[2]	郅军锐, 田甜, 温娟, 刘勇. 西花蓟马或二斑叶螨为害的菜豆对两者间后取食者体内保护酶和解毒酶活性的影响[J]. , 2016, 59(7): 707-715.
[3]	陈伟, 徐卫华. 棉铃虫转录因子c-Myc基因在滞育和非滞育蛹脑中的表达分析及多克隆抗体制备[J]. , 2015, 58(2): 115-121.
[4]	刘建业, 钱蕾, 蒋兴川, 和淑琪, 李正跃, 桂富荣. CO₂浓度升高对西花蓟马和花蓟马成虫体内解毒酶和保护酶活性的影响[J]. , 2014, 57(7): 754-761.
[5]	王梦龙，朱未，张继红，徐安英，王红托, 李瑄，周桂灵，张寰，秦启联. 氧化损伤是热胁迫下小金蝠蛾幼虫不能存活的重要原因[J]. , 2014, 57(7): 769-776.
[6]	孙猛，陆明星，汤小天，杜予州. 大螟HSC70基因克隆及表达模式分析[J]. , 2014, 57(7): 787-797.
[7]	岳雷，周忠实，刘志邦，郭建英，万方浩. 不同强度快速冷驯化对广聚萤叶甲成虫耐寒性生理指标的影响[J]. , 2014, 57(6): 631-638.
[8]	杨光平, 刘玉娣, 侯茂林. 二化螟滞育幼虫的蛋白和核酸含量以及保护酶活性的变化[J]. , 2013, 56(3): 251-256.
[9]	卢增斌，韩乃顺，徐刚，刘玉娥，胡萃，彭于发，郭予元，叶恭银. 转cry1Ab/vip3H+epsps基因粳稻对非靶标害虫稻叶蝉田间种群的影响[J]. , 2013, 56(11): 1275-1285.
[10]	田雨浓, 马伟, 韦庆慧, 罗帅, 韩旭洋, 陈旭日, 邱本军, 马玲. α-萜品醇熏蒸对大麦虫体内抗氧化酶活性的影响[J]. , 2013, 56(1): 47-53.
[11]	王兴云, 马文静, 韩兰芝, 侯茂林. 大螟中肠氨肽酶N基因的克隆及表达谱分析[J]. , 2012, 55(9): 1022-1030.
[12]	解娜, 江幸福, 罗礼智, 张蕾. Cry1Ac杀虫蛋白对粘虫中肠几种酶活性的影响[J]. , 2012, 55(2): 168-175.
[13]	毕明娟, 薛明, 李庆亮, 王洪涛, 刘爱红. 取食B型烟粉虱前侵染的烟草对B型烟粉虱和烟蚜体内保护酶和消化酶活性的影响[J]. , 2010, 53(2): 139-146.
[14]	周利琳, 司升云, 汪钟信, 望勇, 刘小明. 甜菜夜蛾对虫酰肼抗性的生化机制[J]. , 2009, 52(4): 386-394.
[15]	司树鼎，王开运，张文成，王冕，石绪根，栾炳辉. 虫酰肼及其衍生物0593对家蚕的毒性及作用机理[J]. , 2008, 51(11): 1157-1163.