关键词: 黄胫小车蝗, 地理种群, 线粒体COI基因, 单倍型, 遗传分化, 基因流

Abstract: Oedaleus infernalis Saussure is one of the most important agricultural and grass forage pests, which is widely spread in China. The partial sequences of mtDNA COI gene in 10 geographic populations of O. infernalis were sequenced and analyzed. The genetic diversity, genetic differentiation, gene flow and molecular variance were analyzed using DnaSP 5.0 and Arlequin 3.5.1.2. Bayesian inference (BI) phylogenetic tree and the haplotype network of COI haplotypes were constructed. The results showed that a total of 21 haplotypes were defined among 144 gene sequences, including one haplotype shared by ten populations. The total haplotype diversity of all populations was 0.653 and the range of haplotype diversity of different populations was 0.423-0.790. The Tajima’s D test indicated that there might be no population expansion for the total in recent history. The total Gst was 0.04436, Fst was 0.05255 and the gene flow Nm was 9.01 among 10 populations. Molecular variance analysis (AMOVA) demonstrated that observed genetic differentiation mainly occurs within populations and just a little differentiation exists among populations. The genetic distance among populations was not correlated with the geographic distribution. The Bayesian inference (BI) phylogenetic tree and haplotype network showed that haplotypes were distributed in different clades and no obvious geographical structure formed. The results suggest that the gene flow among O. infernalis populations is not affected by the geographical distance.

Key words: Oedaleus infernalis, geographic population, mtDNA COI gene, haplotype, genetic differentiation, gene flow