关键词: 大豆蚜, 热休克蛋白, 吡虫啉, 热激, mRNA表达

Abstract: 【Aim】 Insects will express heat shock proteins (HSPs) efficiently to establish self-protection mechanism under high temperature and pesticide stress. This study aims to know the molecular mechanisms of hsp70 and hsc70 mRNA expression in Aphis glycines under thermal and imidacloprid stress at the transcriptome level and look for the vulnerable spot, so to provide some theoretical basis for biological control. 【Methods】 We adopted the homology-based cloning, RACE technique and real-time fluorescent quantitative PCR methods to research the expression of hsp70 and hsc70 in the 4th instar nymphs of A. glycines at different heat shock time or different recovery time after heat shock and after treatment with different concentrations of imidacloprid. 【Results】 The hsp70 transcription level in the 4th instar nymphs of A. glycines increased first after heat shock at 37℃, reaching 10.36-fold as high as that of the control (P<0.05) at 1 h after heat shock, and then declined little by little. The recovery time after heat shock significantly influenced the hsp70 expression quantity. The hsp70 of A. glycines was abundantly expressed immediately after heat shock, with the expression level of 8.78-fold as high as that of the control (P<0.05), and then decreased to the control level, while the hsc70 expression quantity did not change significantly (P>0.05). Treated with imidacloprid, the peak time of hsp70 and hsc70 expression quantity in the 4th instar nymphs of A. glycines varied with the concentration of imidacloprid and stress time, showing an obvious short-term effect with a downward trend after rising first. 【Conclusion】 The transcription level of hsp70 and hsc70 in A. glycines under imidacloprid stress increases. However, hsp70 and hsc70 in A. glycines subjected to heat shock have different transcription expression patterns: the high temperature induces the expression of hsp70, but has no obvious inductive effect on hsc70.

Key words: Aphis glycines, heat shock protein (HSP), imidacloprid, heat shock, mRNA expression