关键词: 黑水虻, 蛋白质提取, 碱提法, 酶提法, 盐提法, Tris-HCl缓冲液提法, 响应面, 工艺优化

Abstract: 【Aim】 To compare four different extraction methods of proteins from larvae of the black soldier fly, Hermetia illucens, choose the method with highest extraction rate, and obtain the optimum process parameters so as to provide reference to protein extraction and resource utilization of H. illucens. 【Methods】 The methods of extraction by lye, enzyme, salt and Tris-HCl buffer were used and comparatively analyzed in extracting proteins from larvae of the black soldier fly. Then, the optimum levels of NaOH mass concentration, liquid-to-solid ratio, extraction temperature and time were determined by means of single factor tests. The experiment and analysis of Box-Behnken response surface was conducted based on the results of univariate tests and the actual situation. 【Results】 Among the four extraction methods of proteins from black soldier fly larvae, lye extraction was optimal, with the optimum extraction conditions of NaOH mass concentration 2.44 g/100 mL, liquid-to-solid ratio 22 mL/g, extraction temperature 53℃ and extraction time 2 h. The corresponding extraction ratio of verification test was 88.49%, with the relative error of only 0.28% compared with predictive value. 【Conclusion】 The response surface model is good fit, and the optimum extraction process is feasible.

Key words: Hermetia illucens, protein extraction, lye extraction, enzyme extraction, salt extraction, Tris-HCl buffer extraction, response surface methodology, process optimization