喷施吲哚-3-乙酸 (IAA) 对番茄及其上B型烟粉虱的影响

›› 2014, Vol. 57 ›› Issue (7): 824-830.

喷施吲哚-3-乙酸 (IAA) 对番茄及其上B型烟粉虱的影响

邸宁^1,#, 张献忠^2,#, 张凯¹, 张世泽¹, 刘同先^1,*

（1. 西北农林科技大学，西北旱区作物逆境生物学国家重点实验室，农业部西北黄土高原作物有害生物综合治理重点实验室，陕西杨凌 712100； 2. 山东淄博高青农业局，山东淄博 256300）

出版日期:2014-07-20 发布日期:2014-07-20

Influences of indole-3-acetic acid (IAA) spraying on Solanum lycopersicum plants and the infesting Bemisia tabaci (Gennadius) biotype B (Hemiptera: Aleyrodidae) whiteflies

DI Ning^1,#, ZHANG Xian-Zhong^2,#, ZHANG Kai¹, ZHANG Shi-Ze¹, LIU Tong-Xian^1,*

(State Key Laboratory of Crop Stress Biology for Arid Areas, and Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture, Northwest A＆F University, Yangling, Shaanxi 712100, China; 2. Agricultural Bureau of Gaoqing, Zibo, Shangdong 256300, China)

Online:2014-07-20 Published:2014-07-20

摘要/Abstract

摘要： 【目的】不同植物生长调节物质对植食性昆虫产卵、发育、取食等常有促进或抑制作用，进而影响植物与昆虫的互作关系。本研究旨在阐明常用的植物生长调节剂吲哚-3-乙酸对B型烟粉虱Bemisia tabaci (Gennadius)的影响，进而探索防治烟粉虱环境友好的方法，同时期望获得更加优质的烟粉虱用作替代寄主饲养寄生蜂。【方法】对番茄 Solanum lycopersicum L.植株外源喷施吲哚-3-乙酸(IAA)，检测其生理生化的响应；记录B型烟粉虱存活率、产卵量并测量解毒酶等指标。【结果】喷施IAA后番茄叶片含水量略有上升，叶面积增大，叶绿素含量增加，过氧化物酶(POD)活性降低，多酚氧化酶(PPO)活性升高，超氧化物歧化酶(SOD)活性变化不显著(P＞0.05)。喷施IAA后番茄上B型烟粉虱成虫存活率高于对照组，其中50 mg/L IAA番茄上B型烟粉虱成虫存活率和总产卵量最高，250 mg/L IAA存活率最低；喷施IAA 120 h后烟粉虱总产卵量升高；碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)活性较对照升高。【结论】喷施不同浓度的IAA溶液后，番茄植株的生理生化水平发生不同程度的改变，烟粉虱成虫的存活状况、产卵量以及体内解毒活性也会产生不同程度的改变。适宜剂量的IAA处理能够使烟粉虱的生存表现更加优异，从而为饲养寄生蜂提供优质的替代寄主。

关键词: B型烟粉虱, 吲哚-3-乙酸(IAA), 番茄, 解毒酶, 存活率, 产卵

Abstract: 【Aim】 Different plant growth regulators could either facilitate or inhibit herbivores’ oviposition behavior, development and foraging, thus affecting the relationship between insects and their host plants. By detecting the potential effects of the widely used plant growth regulator indo-3-acetic acid (IAA) on Bemisia tabaci (Gennadius) biotype B, we aim to find out environmentally-friendly methods to whitefly management and hope to rear better whiteflies as alternative host of parasitoids. 【Methods】 We sprayed IAA on tomato Solanum lycopersicum L. seedlings, and then measured the physiological indexes of tomato leaves and the influences of IAA on the survival, oviposition and detoxification enzyme activities of B. tabaci adults. 【Results】 After application of IAA, the water content, leaf area, polyphenol oxidase (PPO) activity and chlorophyll concentration in tomato leaves increased, the peroxidase (POD) activity decreased, and the superoxide dismutase (SOD) activity did not show significant change (P＞0.05). B. tabaci adults had a higher survival rate and deposited more eggs on the plants treated with IAA. The survival rate of B. tabaci adults and the total number of eggs laid were the greatest in the 50 mg/L IAA treatment, and the survival rate of B. tabaci adults was the lowest in the 250 mg/L IAA treatment. The alkaline phosphatase (AKP) and SOD activities were higher in the treatment groups than in the untreated controls. 【Conclusion】 After tomato plants are sprayed with different concentrations of IAA solution, the physiological and biochemical parameters of tomato change to different degrees, and the survival, oviposition and detoxification enzyme activities of whiteflies on the plants also change to one degree or another. Appropriate doses of IAA could facilitate better performance of whiteflies, by which we can provide more excellent substitutable hosts for breeding wasps.

Key words: Bemisia tabaci B type, indole-3-acetic acid （IAA）, tomato, detoxification enzyme, survival rate, oviposition

邸宁, 张献忠, 张凯, 张世泽, 刘同先. 喷施吲哚-3-乙酸 (IAA) 对番茄及其上B型烟粉虱的影响[J]. , 2014, 57(7): 824-830.

DI Ning, ZHANG Xian-Zhong, ZHANG Kai, ZHANG Shi-Ze, LIU Tong-Xian. Influences of indole-3-acetic acid (IAA) spraying on Solanum lycopersicum plants and the infesting Bemisia tabaci (Gennadius) biotype B (Hemiptera: Aleyrodidae) whiteflies[J]. , 2014, 57(7): 824-830.

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