外源激素处理后家蚕吡哆醛激酶和磷酸吡哆醇氧化酶转录水平的变化

摘要/Abstract

摘要： 【目的】研究家蚕 Bombyx mori 经蜕皮激素(20-hydroxyecdysone, 20-E)和保幼激素类似物(juvenile hormone analogue, JHA)处理后引起吡哆醛激酶(pyridoxal kinase, PLK)和磷酸吡哆醇氧化酶(pyridoxine-5′-phosphate oxidase, PNPO)的转录水平变化，为进一步研究激素对蚕体营养代谢等工作奠定基础。【方法】以20-E和JHA分别喂食不同发育时期(5龄第1, 3和5天)的家蚕幼虫，以喂食蒸馏水的家蚕为对照，采用实时荧光定量PCR(real-time quantitative PCR)方法在处理后24 和48 h对各组幼虫后部丝腺中PLP合成酶PLK和PNPO的转录水平进行分析。【结果】5龄第1天幼虫经20-E处理24和48 h后，PLK和PNPO的转录水平出现上调且与对照的差异达到极显著 (P＜0.01)；5龄第3天幼虫经20-E处理，PLK的转录水平在48 h出现下调且与对照的差异达到显著(P＜0.05)，PNPO的转录水平在24 和48 h均出现上调且与对照的差异达到极显著 (P＜0.01)；5龄第5天幼虫经20-E处理后PLK和PNPO的转录水平无变化。5龄第1天幼虫经JHA处理后PLK和PNPO的转录水平未受到影响；5龄第3天幼虫经JHA处理后，PLK的转录水平在48 h出现显著下调且与对照的差异达到显著(P＜0.05)，PNPO的转录水平在24和48 h后均出现显著下调且与对照的差异达到极显著(P＜0.05)；5龄第5天幼虫经JHA处理24和48 h后，PLK和PNPO的转录水平出现下调且与对照的差异达到极显著 (P＜0.01)。【结论】20-E和JHA显著影响家蚕5龄幼虫PLK和PNPO的转录水平，20-E提高5龄前期家蚕PLK和PNPO的转录水平，JHA降低5龄后期它们的转录水平，为深入研究激素对VB₆的调控奠定基础。

关键词: 家蚕, 蜕皮激素, 保幼激素类似物, 吡哆醛激酶, 磷酸吡哆醇氧化酶, 转录水平

Abstract: 【Aim】 To further research the hormone regulation of nutrition metabolism, we analyzed the transcription levels of pyridoxal kinase (PLK) and pyridoxine-5′-phosphate oxidase (PNPO) of the silkworm, Bombyx mori, treated with 20-hydroxyecdysone (20-E) and juvenile hormone analogue (JHA). 【Methods】 The day-1, day-3 and day-5 5th instar larvae of B. mori were fed with 20-E or JHA, and those fed on distilled water were used as the control. We assayed the transcriptional levels of PLK and PNPO in silk glands of the treated larvae at 24 and 48 h post treatment by using real-time quantitative PCR.【Results】 In the day-1 5th instar larvae treated with 20-E for 24 and 48 h, respectively, the transcriptional levels of PLK and PNPO significantly increased compared with the control (P<0.01). In the day-3 5th instar larvae treated by 20-E, the transcriptional level of PLK decreased compared with the control at 48 h after treatment (P<0.05), whereas the transcriptional level of PNPO significantly increased compared with the control at 24 and 48 h after treatment (P<0.01). In the day-5 5th instar larvae treated with 20-E, the transcriptional levels of PLK and PNPO had no apparent change. In the day-1 5th instar larvae treated with JHA, the transcriptional levels of PLK and PNPO had no change; in the day-3 5th instar larvae treated with JHA, however, the transcriptional level of PLK decreased compared with the control at 48 h after treatment (P<0.05), and the transcriptional level of PNPO decreased compared with the control at 24 and 48 h after treatment (P<0.01). In the day-5 5th instar larvae treated with JHA for 24 and 48 h, respectively, the transcriptional levels of PLK and PNPO significantly decreased compared with the control (P<0.01). 【Conclusion】 The transcriptional expression of PLK and PNPO is influenced by 20-E and JHA, 20-E promotes them in the early stage of the 5th instar larvae while JHA represses them in late stages of the 5th instar larvae. These results lay the foundation for further research on hormone regulation of VB₆.

Key words: Bombyx mori, 20-hydroxyecdysone, juvenile hormone analogue, pyridoxal kinase, pyridoxine-5′-phosphate oxidase, transcriptional level

杨欢欢, 姚丽丽, 张剑韵, 黄龙全. 外源激素处理后家蚕吡哆醛激酶和磷酸吡哆醇氧化酶转录水平的变化[J]. , 2015, 58(12): 1285-1290.

YANG Huan-Huan, YAO Li-Li, ZHANG Jian-Yun, HUANG Long-Quan. Changes in the transcriptional levels of pyridoxal kinase and pyridoxine-5′-phosphate oxidase post exogenous hormone treatment in the silkworm, Bombyx mori[J]. , 2015, 58(12): 1285-1290.

[1]	朱峰, 高建华, 张永红, 肖圣燕, 邵榆岚, 唐芬芬, 白兴荣. 家蚕微孢子虫感染对家蚕海龟蛋白(Bmtutl)基因表达水平的影响[J]. 昆虫学报, 2019, 62(8): 930-936.
[2]	申雅文, 马田田, 霍春月, 杨宗霖, 路一平, 阚云超, 李丹丹. 家蚕snoRNA Bm-15的细胞定位及与类Notch受体基因的互作关系[J]. 昆虫学报, 2019, 62(8): 895-900.
[3]	路一平, 霍春月, 马田田, 杨宗霖, 申雅文, 阚云超, 李丹丹. 利用体腔显微注射法实现非编码RNA在家蚕头部稳定过表达[J]. 昆虫学报, 2019, 62(7): 779-786.
[4]	张淑尧, 戴易晨, 吴坤钟, 李暑燕, 曹阳, 邓小娟, 田铃. 蜕皮激素对家蚕脂肪体中BmATG8蛋白亚细胞定位与细胞核皱缩的调控[J]. 昆虫学报, 2019, 62(5): 527-534.
[5]	顾健健, 刘红玲, 李凯荣, 孟紫旺, 王慧娟, 沈关望, 张宇靖, 阮杨, 林英, 夏庆友. 用于细胞水平家蚕转录调控元件研究的简单基础启动子的构建[J]. 昆虫学报, 2019, 62(4): 391-397.
[6]	黄燕玲, 黄诗怡, 李树强, 何艳影, 陈泽敏, 刘吉升. 家蚕幼虫BmToll9基因对肽聚糖和金黄色葡萄球菌的响应表达[J]. 昆虫学报, 2019, 62(4): 398-406.
[7]	姚雪, 李琳红, 魏纪珍, 席玉强, 杜孟芳, 崔江宽, 安世恒, 刘晓光. 棉铃虫酪氨酸羟化酶基因的分子特性及功能分析[J]. 昆虫学报, 2019, 62(3): 294-303.
[8]	龙诗慧, 李瑜, 田铃, 李胜, 李康. 家蚕幼虫-蛹变态期前胸腺和脂肪体细胞解离和程序性细胞死亡的比较分析[J]. 昆虫学报, 2019, 62(11): 1239-1249.
[9]	石敏, 李冠楠, 郑茜, 刘凤丹, 赵珊, 蔡苗, 朱勇. 乐果对家蚕生殖腺及氧化应激反应的影响[J]. 昆虫学报, 2019, 62(10): 1186-1196.
[10]	崔勇, 朱亚楠, 黄悦莹, 谭丽庄, 冯启理, 王文, 相辉. 转录因子ZnF-706在鳞翅目昆虫中的进化格局及在家蚕中的功能[J]. 昆虫学报, 2019, 62(1): 9-20.
[11]	郝长富, 李刚, 孙熙, 唐健, 钱荷英, 赵国栋, 邓祥元, 徐安英. 不同饲料饲育的家蚕幼虫肠道细菌的多样性分析[J]. 昆虫学报, 2019, 62(1): 61-72.
[12]	张祥乐, 马俐, 马倩, 李胜, 刘素宁. Ras信号通路通过激活转录因子Myc促进核内复制细胞生长[J]. , 2018, 61(8): 885-894.
[13]	孟竹, 文茂羽, 康晓丽, 卢忠燕, 赵萍, 夏庆友. 饥饿胁迫对家蚕糖脂代谢的影响及BmFoxO的作用[J]. , 2018, 61(8): 895-904.
[14]	殷娅茹, 胡建, 胡文波, 杨成飞, 王坤, 刘春, 林英, 朱勇, 王凌燕. 家蚕油蚕oc突变体突变基因的精细定位[J]. , 2018, 61(6): 627-636.
[15]	杜晓燕, 付开赟, 徐晴玉, 吐尔逊·阿合买提, 丁新华, 何江, 郭文超. 马铃薯甲虫N-β-丙酰多巴胺水解酶基因的克隆及功能分析[J]. , 2018, 61(6): 637-645.

外源激素处理后家蚕吡哆醛激酶和磷酸吡哆醇氧化酶转录水平的变化

Changes in the transcriptional levels of pyridoxal kinase and pyridoxine-5′-phosphate oxidase post exogenous hormone treatment in the silkworm, Bombyx mori

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10