关键词: 意大利蜜蜂')">意大利蜜蜂, 白垩病, 染色体DNA, 非编码区, SNP, 抗病个体

Abstract: 【Aim】 This study aims to screen and verify the SNPs associated with chalkbrood resistance in the non-coding regions of DNA of chromosomes of Apis mellifera ligustica. 【Methods】 Ascosphaera apis spores were inoculated to the artificially reared 3-day-old larvae of A. m. ligustica, and the disease-resistant individuals and susceptible larvae were screened based on whether they displayed typical symptoms. According to the SNP information associated with chalkbrood resistance in the non-coding regions of DNA of chromosomes 2 and 11 from our previous resequencing results, 55 SNPs associated with chalkbrood resistance located in the non-coding regions of DNA of chromosomes 2 and 11 of A. m. ligustica larva were screened and validated by sequencing of PCR products. 【Results】 The results revealed that the SNP T14570310C located in the 5′ non-coding region of the LOC100578413 gene on chromosome 11 of A. m. ligustica showed a higher T allele frequency than the C allele frequency in the disease-resistant individuals and a significantly higher T allele frequency in the disease-resistant individuals than in the susceptible larvae, suggesting that this SNP is associated with the resistance to chalkbrood disease. This molecular marker was consistent with the SNP C2587245T located in the coding region screened previously in respect of the distinction between the disease-resistant and susceptible individuals. 【Conclusion】 The SNP T14570310C in the non-coding region of DNA of chromosome 11 of A. m. ligustica was screened and verified to be associated with the resistance to chalkbrood disease. This SNP provides a new molecular marker for molecular-assisted breeding against chalkbrood disease, and has important significance in early detection of chalkbrood infection and breeding bees resistant to chalkbrood disease.

Key words: Apis mellifera ligustica')">Apis mellifera ligustica, chalkbrood disease, chromosomal DNA, non-coding region, SNP, disease-resistant individuals