昆虫学报 ›› 2019, Vol. 62 ›› Issue (7): 814-822.doi: 10.16380/j.kcxb.2019.07.004

• 研究论文 • 上一篇    下一篇

杨小舟蛾雌蛾性信息素活性成分的鉴定

刘福1, 郭丽2, 李青春3, 张苏芳1, 孔祥波1, 张真1,*, 黄范全3   

  1. (1. 中国林业科学研究院森林生态环境与保护研究所, 国家林业和草原局森林保护学重点实验室, 北京 100091; 2. 邢台学院生物科学与工程学院, 河北邢台 054001; 3. 沅江市林业局, 湖南沅江 413100)
  • 出版日期:2019-07-20 发布日期:2019-07-09

Identification of the active components of sex pheromone of female moths of Micromelalopha sieversi (Lepidoptera: Notodontidae)

LIU Fu1, GUO Li2, LI Qing-Chun3, ZHANG Su-Fang1, KONG Xiang-Bo1, ZHANG Zhen1,*, HUANG Fan-Quan3   

  1. (1. Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China; 2. Academy of Biological Science and Engineering, Xingtai University, Xingtai, Hebei 054001, China; 3. Forestry Bureau of Yuanjiang City, Yuanjiang, Hunan 413100, China)
  • Online:2019-07-20 Published:2019-07-09

摘要:

【目的】鉴定杨小舟蛾Micromelalopha sieversi雌蛾性信息素活性成分的结构信息。【方法】采用正己烷浸提的方法提取杨小舟蛾性成熟处女雌蛾性腺中的活性成分;利用气相色谱-触角电位联用(GC-EAD)技术对其活性成分进行定位;性腺提取物与4-甲基-1,2,4-三唑啉-3,5-二酮(MTAD)进行微量化学反应,获得衍生物;利用气相色谱-质谱联用(GC-MS)技术分别对性腺提取物及MTAD衍生物进行质谱特征离子分析。【结果】GC-EAD结果显示,杨小舟蛾雄蛾触角对雌蛾性信息素腺体提取物中的一种成分有较好的反应;GC-MS分析结果表明,能引起雄蛾触角电生理反应的成分为十八碳的不饱和醛;MTAD衍生物的GC-MS结果显示,该活性成分的两个双键分别位于碳链的13和15位。【结论】本研究鉴定出杨小舟蛾雌蛾性信息素活性成分的平面结构为13,15-十八碳二烯醛,但双键的立体构型有待合成标准化合物进一步鉴定。本研究为杨小舟蛾性信息素备选化合物的筛选提供了方向,为信息素的结构确证奠定了基础。

关键词: 杨小舟蛾, 性信息素, 活性成分, MTAD衍生物, 结构鉴定, 气相色谱-触角电位联用, 气相色谱-质谱联用

Abstract:  【Aim】 This study aims to identify the structure of active components of sex pheromone of female moths of Micromelalopha sieversi. 【Methods】 The sex pheromone was extracted from sex glands of virgin female moths of M. sieversi by n-hexane. The active ingredient was located by gas chromatography-electroantennographic detection (GC-EAD). The additive derivative was obtained by the reaction of the extract from sex glands of virgin female moths with 4-methyl-3H-1,2,4-triazole-3,5(4H)-dione (MTAD). The characteristic ions of the extract and additive derivative were analyzed by gas chromatography-mass spectrometry (GC-MS). 【Results】 The GC-EAD results indicated that the antenna of male moth of M. sieversi showed sensitive reaction to one component of the extract from sex glands of virgin female moths. Based on the GC-MS data of the extract and additive derivative, the active component of sex pheromone was identified as 13,15-octadecadienal. 【Conclusion】 The identified planar structure of the active component of sex pheromone of female moths of M. sieversi is 13,15-octadecadienal, but the stereoscopic configuration of conjugated double bonds needs to be further identified through synthesizing more standard compounds. This study provides the direction in screening synthetic pheromone candidates and establishes the foundation for determining the sex pheromone structure of female moth of M. sieversi.

Key words: Micromelalopha sieversi, sex pheromones, active component, MTAD derivative, structural identification, gas chromatography-electroantennographic detection (GC-EAD), gas chromatography-mass spectrometry (GC-MS)