昆虫学报 ›› 2021, Vol. 64 ›› Issue (3): 409-418.doi: 10.16380/j.kcxb.2021.03.013

• 研究论文 • 上一篇    下一篇

安荔赤眼蜂在中国野外首次发现及其体内Wolbachia的检测

肖庄婷, 王德森, 何余容*   

  1. (华南农业大学昆虫学系, 广东省生物农药创制与应用重点实验室, 生物防治教育部工程技术研究中心, 广州 510642)
  • 出版日期:2021-03-20 发布日期:2021-04-20

First discovery of Trichogramma oleae (Hymenoptera: Trichogrammatidae) in the wild in China and detection of its endosymbiont Wolbachia

XIAO Zhuang-Ting, WANG De-Sen, HE Yu-Rong*   

  1. (Key Laboratory of BioPesticide Innovation and Application, Guangdong Province, Engineering Research Center of Biological Control, Ministry of Education, Department of Entomology, South China Agricultural University, Guangzhou 510642, China)
  • Online:2021-03-20 Published:2021-04-20

摘要: 【目的】为丰富赤眼蜂Trichogramma的种类资源,明确野外新采集的一种赤眼蜂的种类,探明该赤眼蜂所感染Wolbachia的类型。【方法】采用挂米蛾Corcyra cephalonica卵卡法在华南农业大学树木园诱集到两批赤眼蜂,通过形态鉴定和PCR扩增ITS2序列并测序分析的分子鉴定手段对野外采集的赤眼蜂材料进行种类鉴定;通过PCR扩增Wolbachia的外膜蛋白基因(wsp)序列,检测赤眼蜂体内Wolbachia的感染情况;通过PCR扩增wsp序列和多位点序列分型(multilocus sequence typing, MLST)对检测到的赤眼蜂体内的Wolbachia进行同源性分析。【结果】所诱集到的两批赤眼蜂均被鉴定为安荔赤眼蜂Trichogramma oleae Voegelé & Pointel,体内Wolbachia的感染率达100%。该Wolbachia株系与安荔赤眼蜂(前南斯拉夫品系)、短管赤眼蜂T. pretiosum(乌拉圭品系)以及T. deion(荷兰品系)体内Wolbachia亲缘关系较近,属于B超组Sib亚组,对应MLST序列型为ST486。【结论】安荔赤眼蜂T. oleae为中国野外首次发现,是完全感染Wolbachia的产雌孤雌生殖品系。本研究为害虫生物防治提供了一种新的天敌种类资源,并为进一步探明Wolbachia与赤眼蜂的互作提供了研究材料。

关键词: 安荔赤眼蜂, 雄性外生殖器, ITS2, Wolbachia, MLST, 系统发育, 中国

Abstract:

【Aim】 This study aims to determine the species identity of one Trichogramma species recently collected in the field and to identify the types of Wolbachia in the collected Trichogramma populations in order to find more high quality species resources of Trichogramma. 【Methods】 Two atches of Trichogramma wasps were trapped by hanging up egg cards of the rice moth, Corcyra cephalonica, in the Arboretum of South China Agricultural University. Combined with the morphological identification and the molecular identification method through PCR amplification, sequencing and analysis of ITS2 sequence, the species of Trichogramma materials collected in the field were identified. Wolbachia infection in the Trichogramma wasps was detected by PCR amplification of the outer membrane protein gene (wsp) sequence in Wolbachia. The homology analysis of the detected Wolbachia in the Trichogramma wasps was carried out by PCR amplification of wsp and multilocus sequence typing (MLST). 【Results】 All the two batches of Trichogramma wasps collected in this study were identified as Trichogramma oleae Voegelé & Pointel, in which the infection rate of Wolbachia was 100%. The results showed that Wolbachia in T. oleae belongs to the Sib subgroup in the B supergroup, corresponding to MLST sequence type ST486, having the close relationship with the Wolbachia in T. oleae (former Yugoslavia line), T. pretiosum(Uruguay line) and T.deion (Netherlands line). 【Conclusion】 This is the first record of T. oleae captured in the wild in China, which is a parthenogenetic strain with complete infection of Wolbachia. This study provides a new natural enemy species resource for pest biological control and a valuable research material for further study on the interaction between Wolbachia and Trichogramma.

Key words: Trichogramma oleae, male external genitalia, ITS2, Wolbachia, MLST, phylogeny, China