›› 1993, Vol. 36 ›› Issue (3): 277-281.

• 研究论文 • 上一篇    下一篇

长管蚜激脂激素提取物生物活性的研究

吴振廷 R.E.Isaac   

  • 出版日期:1993-08-20 发布日期:1993-08-20

BIOLOGICAL ACTIVITIES OF ADIPOKINETC HORMONEEXTRACT FROM AN APHID, MACROSIPHUM SP.

Wu ZHEN-TING R. E. ISAAC   

  • Online:1993-08-20 Published:1993-08-20

摘要: 激脂激素和高血糖激素已在某些昆虫种类中发现,但是到目前为止还未涉及到同翅目昆虫。本研究中,从同翅目昆虫长管蚜Mcrosiphum sp.体内分离了激脂激素提取物,并进行了生物活性测定。结果证明,这种提取物具有很高的生物活性,注射20毫克蚜虫重的提取物,能使试虫沙漠蝗与美洲蜚蠊体液中脂肪含量和糖含量分别增加131.03%和60.43%。 试验结果还展示出长管蚜激脂激素提取物激脂效应要比高血糖效应高得多,它们的生物活性能被内肽酶24∶11,焦谷氨酰胺基肽酶和沸水浴抑制或破坏。

关键词: 长管蚜虫, 沙漠蝗, 美洲蜚蠊, 激脂激素

Abstract: Adipokinetic and hyperglycemic hormones have been discovered in several insects, but no approach has been made to homopterans. In this study, crude extract of adipokinetic hormone was isolated from an aphid, Macrosiphum sp., and its biological activities were tested. Infecuon of the crude extract equivalent to 20 mg aphids mto Schistocerca gregaria and Pertptaneta americana caused increases of lipid and sugar levels in haemolymph by 131.03% and 60.43%, respectively. The results showed that the extract had a much higher adlpokinetic activity than hyperglycemic effect. The activities could be suppressed by treatment with endopeptidase, pyroglptamyl aminopeptidae or high temperature.

Key words: Macrosiphum sp——Schistocerca gregaria——Periplaneta americana