›› 1993, Vol. 36 ›› Issue (4): 390-395.

• 研究论文 • 上一篇    下一篇

刀豆氨酸对亚洲玉米螟的生理生化效应

程振衡 汪文陆   

  • 出版日期:1993-11-20 发布日期:1993-11-20

PHYSIOLOGICAL AND BIOCHEMICAL EFFECTS OFL-CANAVANINE ON THE CORN BORER,OSTRINIA FURNACALIS GUENEE

CHENG ZHEN-HENG WANG WEN-LU   

  • Online:1993-11-20 Published:1993-11-20

摘要: 本文报道亚洲玉米螟Ostrinia furnacalis五龄幼虫注射0.25-1.0毫克/克刀豆氨酸48小时后有关生理生化的变化,其中血淋巴蛋白质电泳区带数目减少,浓度降低,而游离氨基酸总量明显增加,其中脯氨酸和谷氨酸的变化最为明显。虫体酸性磷酸酯酶的活性显著升高,而精氨酸酶的活性却有所降低。刀豆氨酸可引起虫体内尿素含昆下降,而对尿酸含量影响不大。经刀豆氨酸处理后发育成长的成虫其雌虫卵巢和雄虫附腺的蛋白质合成均显著下降,并对上述结果做了简要的讨论。

关键词: 亚洲玉米螟, 刀豆氨酸, 血淋巴蛋白质, 氨基酸

Abstract: When L-canavanine was injected into the body cavity of Ostrinia furnacalitlarva at doses of 0.25-1.00mg/g body weight, it considerably depressed the amount of hemolymph proteins and significantly increased the level of hemolymph freeamino acids, among which proline and glutamic acid were most markedly affected.The whole-body acid phosphatase activity was elevated, while the whole-body arginase activity and urea concentrations decreased. There was no significant changes in uric acid. Incorporations of3H-leucine into trichloroacetic acid-insoluble substanceswere significantly reduced in the ovary or male accessory glands of adults from larvae treated with canavanine. It was suggested that the toxicity of canavanine was much dependent on the disturbance of protein and nitrogen metabonism, which in turn affects a series of changes of physiological processes.

Key words: Ostrinta furnacalis——L-canavanine——hemolymph proteins—— amino acids