›› 1999, Vol. 42 ›› Issue (3): 248-256.

• 研究论文 • 上一篇    下一篇

抗溴氰菊酯家蝇在不同用药方式下的敏感性变化及其机制

邱立红, 李学锋, 王成菊, 张文吉   

  • 出版日期:1999-08-20 发布日期:1999-08-20

Chanes of susceptibility of resistant housefly to pesticides and it's mechanisms under different insecticide application

Qiu Li-hong,Li Xue-feng,Wang Cheng-ju,Zhang Wen-ji   

  • Online:1999-08-20 Published:1999-08-20

摘要: 以具有极高抗水平的抗溴氰菊酯家蝇Musca domestica vicina Macquart DR0品系为试虫,模拟田间几种常见的用药方式(混用、轮用、使用增效剂),在室内进行平行汰选,并以不用药和继续用原药汰选的为比较,研究试虫在这几种用药方式下的敏感性变化及其变化机制。抗性家蝇用辛溴混剂、辛硫磷以及溴氰菊酯+SV1汰选后,在F16(F17)代以前,对溴氰菊酯及汰选药剂的抗性发展相对都比较缓慢;F16(F17)代以后,用溴氰菊酯+SV1汰选的家蝇对溴氰菊酯的敏感性迅速下降,抗性发展很快。家蝇对溴氰菊酯的敏感性变化与药剂中溴氰菊酯的选择压有关。生化分析结果表明,在不同用药方式汰选下,家蝇体内酯酶、多功能氧化酶、谷胱甘肽-S-转移酶、乙酰胆碱酯酶的酶活或特性发生了不同的变化。

关键词: 家蝇, 抗性, 溴氰菊酯, 敏感性变化, 用药方式

Abstract: A housefly (Musca domestica vicina Macquart) strain (DR0) possessing high level resistance to deltamethrin was selected under different insecticide applications in the laboratory, involving alternating insecticide(phoxim), using insecticide mixture(1∶100 mixture of deltamethrin and phoxim) and deltamethrin adding synergist(SV1). Before generation F16 (F17),the development of resistance to deltamethrin and selecting insecticides was relatively slow in all three strains; but after that, the rate of development increased, especially that of the deltamethrin+SV1 selected strain to deltamethrin.The changes of susceptibility to deltamethrin were correlated with the selection pressure. Biochemical studies showed that,the changes of enzyme activities or properties of esterase, carboxylesterase, mixed-function oxidase, glutathione-S-transferase and acetylcholinesterase were responsible for the changes of susceptibility in the resistant housefly.

Key words: housefly, resistance, deltamethrin, changes of susceptibility, insecticide application