关键词: 烟蚜, RAPD-PCR, 相似性指数, 遗传距离, 种群分化

Abstract: RAPD-PCR was used to detect the population differentiation of green peach aphid, Myzus persicae (Sulzer) on tobacco from different regions in China, and the results showed that the use of different primers in the RAPD method permits detection of different levels of DNA polymorphism in the aphid's population. Nei's genetic distance (D) shows that the differentiation of the aphid from tobacco is only at population level instead of subspecies or species level. Three primers screened from nineteen were used for cluster analysis based on similarity index (SI) and Nei's genetic distance (D). Although there exist some differences between the cluster results of SI and D, they both can show the DNA differences of geographic populations and color forms of the aphid. At DNA level, compared with the holocyclic aphids (from northern China), the aphids mixed with holocyclic and anholocyclic life cycles (from central China) are more similar to the anholocyclic aphids (from southern China), but there is no obvious difference among the aphids from central China. The results also show that, compared with yellowish green forms, the red forms are more similar to the brown ones of the aphid.

Key words: Myzus persicae (Sulzer), RAPD-PCR, similarity index, genetic distance, population differentiation