›› 2000, Vol. 43 ›› Issue (-1): 70-76.

• 研究论文 • 上一篇    下一篇

繁殖寄主对赤眼蜂羧酸酯酶和乙酰胆碱酯酶的影响

宗静1,张帆2,孙光芝2,李长春2,高希武1*,郑炳宗1   

  • 出版日期:2000-12-20 发布日期:2000-12-20

Author for correspondenceEffects of host eggs on charateristics of carboxylesterase andacetylcholinesterase in Trichogramma

ZONG Jing1,ZHANG Fan2,SUN Guang-zhi2,LI Chang-chun2,GAO Xi-wu1,ZHENG Bing-zong1   

  • Online:2000-12-20 Published:2000-12-20
  • Contact: GAO Xi-wu

摘要: 通过测定赤眼蜂Trichogramma羧酸酯酶和乙酰胆碱酯酶的活性、对底物的亲和力以及对抑制剂的敏感度研究了繁殖寄主对松毛虫赤眼蜂T.dendrolimi和螟黄赤眼蜂T.chilonis的影响。柞蚕卵和米蛾卵繁殖的赤眼蜂羧酸酯酶对底物的亲和力有不同程度的影响,柞蚕卵繁殖的赤眼蜂羧酸酯酶对α-乙酸萘酯或β-乙酸萘酯的亲和力最高是米蛾卵的2倍以上。繁殖寄主对乙酰胆碱酯酶对底物亲和力没有明显的影响。米蛾卵繁殖的松毛虫赤眼蜂羧酸酯酶活性明显高于柞蚕卵繁殖的种群,而米蛾卵繁殖的螟黄赤眼蜂种群羧酸酯酶的活性明显低于柞蚕卵繁殖的种群。用柞蚕卵繁殖的松毛虫赤眼蜂种群对对氧磷的敏感度明显低于米蛾卵繁殖的种群,而增效磷则正好相反。繁殖寄主对松毛虫赤眼蜂吉林种群乙酰胆碱酯酶对DDVP和毒扁豆碱的敏感度没有明显的影响,而在松毛虫赤眼蜂广东种群和螟黄赤眼蜂中,柞蚕卵繁殖的种群乙酰胆碱酯酶对DDVP和毒扁豆碱的敏感度明显低于米蛾卵繁殖的种群。

关键词: 赤眼蜂, 繁殖寄主, 羧酸酯酶, 乙酰胆碱酯酶, 杀虫药剂

Abstract: The effects of host eggs on Trichogramma dendrolimi and T. chilonis were investigated based on comparisons of the activities of carboxylesterase (CarE) and acetylcholinesterase (AChE) and their affinities toward substrates and sensitivities to insecticides. The activities of CarE in T. dendrolimi from Guangdong and Jilin Provinces reared on Antheraea pernyi eggs were lower than those of the same population reared on Corcyra cephalonica eggs, but the activity of CarE in T. chilonis from Hubei Province reared on A. pernyi eggs was higher than that reared on C. cephalonica eggs. The affinity of CarE to α-naphthyl acetate (α-NA) was higher in T. dendrolimi (Guangdong population) reared on A. pernyi eggs than those reared on C. cephalonica eggs. The affinity of AChE in the three Trichogramma populations reared on A. pernyi eggs was lower than that reared on C. cephalonica eggs. Comparing with the Trichogramma population reared on C. cephalonica eggs, the CarE of population reared on A. pernyi eggs was sensitive to SV1, and the sensitivity to paraoxon varied with the populations. The sensitivity of T. dendrolimi (Jilin population) AChE to DDVP and esterine using A. pernyi eggs as hosts was not significantly different from using C. cephalonica eggs as hosts, While, the AChE in T. dendrolimi (Guangdong population) and T. chilonis (Hubei population) reared on C. cephalonica eggs was more sensitive to DDVP and eserine than in those reared on A. pernyi eggs.

Key words: Trichogramma, host egg, carboxylersterase, acetylcholinesterase, insecticide