›› 2001, Vol. 44 ›› Issue (4): 416-421.

• 研究论文 • 上一篇    下一篇

用微卫星引物PCR分析棉蚜不同蚜型的DNA多态性

龚鹏1, 张孝羲1, 杨效文2, 陈晓峰2   

  • 出版日期:2001-11-20 发布日期:2001-11-20

Microsatellite DNA polymorphism in different forms of the cotton aphid

GONG Peng1, ZHANG Xiao-xi1, YANG Xiao-wen2, CHEN Xiaofeng2   

  • Online:2001-11-20 Published:2001-11-20

摘要: 用微卫星引物PCR方法, 对棉蚜Aphis gossypii干母、干雌、孤雌蚜(有翅迁飞孤雌蚜和无翅孤雌蚜)、性母、性雌蚜和雄蚜的DNA多态性进行分析。 结果表明:① 棉蚜的有性蚜型和无性蚜型之间在遗传上有较大的差异; ② 性母与性雌蚜和雄蚜的遗传关系都十分接近,说明同一性母既产性雌蚜,也产雄蚜;③ 干母和干雌的遗传关系很近, 但孤雌蚜已与二者有分化。

关键词: 棉蚜, 蚜型, 微卫星引物, PCR, DNA多态性

Abstract: The PCR technique with microsatellite primers was used to analyze the DNA polymorphism in different forms (mother of mating forms, mating female, male, fundatrix, apterous and alate virginoparae ) of the cotton aphid, Aphis gossypii. The results showed that: 1. There existed a obvious genetic differentiation between sexual forms and parthenogenetic forms of the cotton aphid;2. Mother of mating forms had almost the same genetic relation to the mating female and male, suggesting that the mother of mating female also be the mother of male;3. Compared with virginoparae, the genetic relationship between fundatrix and the first generation of virginoparae (apterous) produced by fundatrix (apterous) was much close.

Key words: Aphis gossypii, forms, microsatellite primers, PCR, DNA polymorphism