›› 2001, Vol. 44 ›› Issue (4): 447-453.

• 研究论文 • 上一篇    下一篇

微粒体多功能氧化酶系与棉铃虫对氰戊菊酯抗药性的关系

邱立红, 张文吉   

  • 出版日期:2001-11-20 发布日期:2001-11-20

Relationship between mixedfunction oxidases and the resistanceto fenvalerate in Helicoverpa armigera

QIU Li-hong, ZHANG Wen-ji   

  • Online:2001-11-20 Published:2001-11-20

摘要: 测定了棉铃虫Helicoverpa armigera抗氰戊菊酯种群及相对敏感种群不同组织微粒体的甲氧试卤灵-O-脱甲基酶、乙氧试卤灵-O-脱乙基酶、乙氧香豆素-O-脱乙基酶、芳烷基羟基化酶和艾氏剂环氧化酶的活性。结果表明:抗性种群棉铃虫中肠组织的这5种酶活性分别比敏感种群的活性提高了11.29、4.10、2.66、6.30和2.34倍,其脂肪体及体壁的相应酶活性则分别为敏感种群的1.46、6.80、1.36、4.05、1.48倍和12.32、2.2、1.33、0.80和0.51倍。两种群中,棉铃虫不同组织部位的各单加氧酶活性均不同,活性高低顺序在两种群间也不同。总体而言,均是中肠或脂肪体微粒体对不同底物的氧化代谢能力最强。

关键词: 棉铃虫, 抗药性, 多功能氧化酶系, 单加氧酶

Abstract: Activities of methoxyresorufin O-demethylase, ethoxyresorufin O-deethylase, ethoxycoumrin O-deethylase, aryl hydrocarbon hydroxylase and aldrin epoxidase were studied in three tissues of a fenvalerate-resistant strain and a susceptible strain of Helicoverpa armigera. Compared with the susceptible strain, activities of the five monooxygenases in the resistant strain increased 11.29-, 4.10-, 2.66-, 6.30-, 2.34-folds respectively in midgut microsomes, 1.46-, 6.80-, 1.36-, 4.05-, 1.48-folds respectively in fat body microsomes and 12.32-, 2.2-, 1.33-, 0.80-, 0.51-folds respectively in integument microsomes. The ability to oxidize the five substrates was different for the three tissues in both strains. Generally, midgut or fatbody microsomes showed the highest monooxygenase activity.

Key words: Helicoverpa armigera, resistance, mixedfunction oxidases, monooxygenase