›› 2002, Vol. 45 ›› Issue (4): 425-429.

• 研究论文 • 上一篇    下一篇

转Bt基因棉挥发性气味的化学成分及其对棉铃虫的电生理活性

阎凤鸣1,2, 许崇任1, Marie BENGTSSON2, Peter WITZGALL3, Peter ANDERSON3   

  • 出版日期:2002-08-20 发布日期:2002-08-20

Volatile compositions of transgenic Bt cotton and their electrophysiological effects on the cotton bollworm

YAN Feng-Ming1,2, XU Chong-Ren1, Marie BENGTSSON3, Peter WITZGALL3,Peter ANDERSON3   

  • Online:2002-08-20 Published:2002-08-20

摘要: 报道了对转Bt基因棉“GK-12”及其常规棉亲本“泗棉3号”挥发性化学物质的分析结果,以及棉铃虫对Bt棉挥发性化学物质的电生理反应,以期为抗虫棉的生态学和安全性评价提供化学生态学的证据。结果表明,在7~8个真叶期,转Bt基因棉的α-蒎烯和β-蒎烯的相对含量比常规棉高许多,倍半萜烯C和一个含量很低的化合物(该化合物对棉铃虫有电生理活性)是常规棉所没有的。转基因Bt棉的挥发性物质中有9个化合物可引起棉铃虫的触角电位反应。

关键词: 挥发性次生物质, 转Bt基因棉, 化学分析, 气相色谱触角电位, 棉铃虫

Abstract: In order to provide chemical and ecological evidence for the ecological risk assessment of transgenic cotton volatile chemical compositions from transgenic Bt cotton “GK-12” and regular cotton “Simian No. 3” were analysed and antennal responses of mated female cotton bollworms to these substances are reported. The ratios of α-pinene and β-0pinene were much higher in the volatiles of transgenic Bt cotton than those of regular cotton and two compounds in transgenic Bt cotton volatiles were absent in regular cotton. Nine compounds in volatiles of transgenic Bt cotton were responsible for EAD peaks in GC-EAD tests.

Key words: volatile secondary metabolites, transgenic Bt cotton, chemical analysis, GCEAD, Helicoverpa armigera