小菜蛾酪氨酸酶生物化学性质研究

›› 2003, Vol. 46 ›› Issue (5): 553-557.doi:

小菜蛾酪氨酸酶生物化学性质研究

梁沛，　孟凤霞，　高希武，郑炳宗，　程刚

出版日期:2003-10-20 发布日期:2003-10-20

Biochemical characteristics of tyrosinase in the diamondback moth, Plutella xy lostella（L.）

LIANG Pei, MENG Feng-Xia, GAO Xi-Wu^*, ZHENG Bing-Zong, CHENG Gang

Online:2003-10-20 Published:2003-10-20
Contact: GAO Xi_Wu

摘要/Abstract

摘要： 采用时间动力学法对小菜蛾Plutella xylostella酪氨酸酶的最适反应条件、对抑制剂的敏感度以及发育期变化规律进行了研究。结果表明：小菜蛾酪氨酸酶反应的最适pH值为6.4，反应线性时间为0～2 min，在22℃和4℃下其活性分别可保持12 h 和72 h，在-20℃下至少可保持7 天。不同发育阶段小菜蛾酪氨酸酶活性的大小依次为预蛹＞4龄幼虫＞蛹＞1龄、2龄、3龄幼虫＞成虫。苯基硫脲（phenyl thiourea，PTU）对小菜蛾阿维菌素抗性品系和敏感品系酪氨酸酶活性的抑制中浓度（I₅₀）值分别为1.1796 μmol/L和1.2795 μmol/L，二者无明显差异。

关键词: 小菜蛾；　酪氨酸酶, 　生物化学性质

Abstract: The optimum reaction conditions, sensitivity to the inhibitor and the developmental changes of tyrosinase in the diamondback moth, Plutella xylostella (L.), were studied using time-driving dynamic method. The results showed that the optimum pH of tyrosinase is about 6.4; The OD₄₂₀ values are linear with time from 0-2 min. When stored at 22℃ and 4℃, the prepared tyrosinase could keep its best activity for 12 h and 72 h, respectively; and while stored at -20℃, the tyrosinase could keep its activity for at least 7 days. The activity of tyrosinase in different developmental stages of P. Xylostella follows the order pharate pupa>4th instar larvae >pupa>1st－3rd instar larvae > adult. The I₅₀ of phenyl thiourea (PTU) to the activity of tyrosinase from both the resistant and susceptible strains showed no obvious difference (1.1796 μmol/L and 1.2795 μmol/L, respectively).

Key words: Plutella xylostella, tyrosinase, 　biochemical c haracteristics

梁沛，　孟凤霞，　高希武，郑炳宗，　程刚. 小菜蛾酪氨酸酶生物化学性质研究[J]. , 2003, 46(5): 553-557.

LIANG Pei, MENG Feng-Xia, GAO Xi-Wu^*, ZHENG Bing-Zong, CHENG Gang. Biochemical characteristics of tyrosinase in the diamondback moth, Plutella xy lostella（L.）[J]. , 2003, 46(5): 553-557.

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