›› 2004, Vol. 47 ›› Issue (1): 20-24.

• 研究论文 • 上一篇    下一篇

昆虫病原线虫斯氏线虫和异小杆线虫对长角血蜱雌蜱的致病力

高志华1,杨小龙1,刘敬泽1*,简恒2   

  • 出版日期:2004-02-20 发布日期:2004-02-20

Virulence of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) to female ticks of Haemaphysalis longicornis Neumann (Acari: Ixodidae)

GAO Zhi-Hua1,YANG Xiao-Long1,LIU Jing-Ze1*,JIAN Heng2   

  • Online:2004-02-20 Published:2004-02-20
  • Contact: LIU Jing-Ze

摘要: 用昆虫病原线虫小卷蛾斯氏线虫(Sc BJ)、夜蛾斯氏线虫(Sf Otio)、拟双角斯氏线虫(Sc D43)、格氏斯氏线虫(Sg NC32)和嗜菌异小杆线虫(Hb E-6-7)对长角血蜱雌蜱进行感染试验,所用线虫剂量为4 000 Ijs/dish。结果表明,5种线虫均对长角血蜱雌蜱有致死效应。Hb E-6-7和Sc BJ两种线虫对雌蜱各发育期致病力最强,导致雌蜱的累积死亡率和半致死时间分别为饥饿雌蜱82.5%,9.0天和75.0%,8.8天;吸血雌蜱90.0%,8.0天和82.5%,8.0天;饱血雌蜱93.3%,7.3天和86.7%,7.3天。Sc D43对饱血雌蜱有较高的致死效应,为80.0%,但半致死时间较长,为11.7天。Sf Otio和Sg NC32对长角血蜱雌蜱的致死效应较低。饱血雌蜱较饥饿雌蜱和吸血雌蜱更易被线虫感染。

关键词: 昆虫病原线虫, 斯氏线虫, 异小杆线虫, 长角血蜱, 致病力

Abstract: Infection of entomopathogenic nematodes Steinernema carpocapsae BJ(Sc BJ),S. feltiae Otio(Sf Otio),S. ceratophorum D43(Sc D43) and S. glaseri NC32(Sg NC32) (Steinernematidae),and Heterorhabditis bacteriophora E-6-7 (Hb E-6-7) (Heterorhabditidae) to female Haemaphysalis longicornis Neumann was conducted. The nematodes dosage used was 4 000 Ijs/dish. The results showed that the five entomopathogenic nematodes had lethal effects on female H.longicornis.Hb E-6-7 and Sc BJ were the most virulence species to variousdevelopmental stages of female ticks: the cumulative mortality and the LT50 of unfed female ticks from nematodes infection were 82.5%,9.0 d and 75.0%,8.8 d, respectively; feeding female 90.0%,8.0 d and 82.5%,8.0 d; engorged female 93.3%,7.3 d and 86.7%,7.3 d. Sc D43 infection also caused high mortality (80.0%) to engorged female ticks, but the LT50 was relatively long (11.7 d). Lethal effects of Sf Otio and Sg NC32 to this tick were low. Engorged female ticks were more sensitive than unfed and feeding female ticks.

Key words: Entomopathogenic nematodes, steinernematid, heterorhabditid, Haemaphysalis longicornis, virulence