绿僵菌侵染小菜蛾体表过程的显微观察

›› 2005, Vol. 48 ›› Issue (2): 188-193.doi:

绿僵菌侵染小菜蛾体表过程的显微观察

王音^1，2, 雷仲仁^2*, 张青文¹, 问锦曾²

中国农业科学院植物保护研究所

出版日期:2005-07-10 发布日期:2005-11-20
通讯作者: 雷仲仁

Microscopic observations of infection process ofMetarhizium anisopliae on the cuticle of the diamondback moth,Plutella xylostella

WANG Yin^1,2, LEI Zhong-Ren^2*, ZHANG Qing-Wen¹,WEN Jin-Zeng²

Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Online:2005-07-10 Published:2005-11-20
Contact: LEI Zhong-Ren

摘要/Abstract

摘要： 采用扫描电镜研究了小菜蛾Plutella xylostella体表结构对绿僵菌入侵行为的影响及绿僵菌的侵染过程。结果表明: 绿僵菌孢子在小菜蛾体表萌发后可形成附着胞，寄主体表结构影响形成附着胞的快慢、多少及穿透体壁时芽管长度, 在平缓结构区和刺状结构区比嵴状结构区更易形成附着胞，且芽管较短。在所有结构区，LF68菌株穿透芽管均短于LD65菌株的芽管。接种后7 h，分生孢子在小菜蛾体表开始萌发，LF68与LD65菌株分别于接种后10 h和13 h出现侵染构造穿透体壁。

关键词: 昆虫病原真菌, 绿僵菌, 小菜蛾, 体表结构, 入侵行为, 侵染过程, 扫描电镜

Abstract: The infection mode of the diamondback moth, Plutella xylostella by two isolates of the entomopathogenic fungus Metarhizium anisopliae was studied using scanning electron microscopy. The results indicated surface topography of host affected infection behavior, especially appressoria production. There were more appressoria formed and shorter germ tubes grew before penetrating in gentle and spinous surface topography than in acanthoid surface topography. The germ tubes of LF68 isolate were shorter than that of LD65 isolate before the appressoria formed or penetration occurred in all kinds of surface topography. Conidia germinated within 7 h postinoculation for the two isolates, but penetrations were observed within 10 h and 13 h for LF68 and LD65 isolates, respectively.

Key words: Entomopathogenic fungus, Metarhizium anisopliae;Plutella xylostella, cuticle topography, invasion behavior, infection process, scanning electron microscopy

王音，雷仲仁，张青文，问锦曾. 绿僵菌侵染小菜蛾体表过程的显微观察[J]. , 2005, 48(2): 188-193.

WANG Yin, LEI Zhong-Ren, ZHANG Qing-Wen, WEN Jin-Zeng. Microscopic observations of infection process ofMetarhizium anisopliae on the cuticle of the diamondback moth,Plutella xylostella[J]. , 2005, 48(2): 188-193.

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