›› 2005, Vol. 48 ›› Issue (6): 859-865.

• 研究论文 • 上一篇    下一篇

浙江B型与非B型(China-ZHJ-1)烟粉虱种群共生细菌的检测及系统发育分析

阮永明,刘树生   

  1. 浙江大学应用昆虫学研究所
  • 出版日期:2005-12-29 发布日期:2005-12-20
  • 通讯作者: 刘树生

Detection and phylogenetic analysis of prokaryotic endosymbionts in Bemisia tabaci

RUAN Yong-Ming, LIU Shu-Sheng   

  1. Institute of Applied Entomology, Zhejiang University
  • Online:2005-12-29 Published:2005-12-20

摘要: 烟粉虱体内存在共生细菌,包括初生共生细菌(primary endosymbiont)和次生共生细菌(secondary endosymbiont)。本项研究应用PCR技术检测了烟粉虱浙江B型和非B型China-ZHJ-1种群中共生细菌的分布。结果表明,烟粉虱B型和非B型体内均存在初生共生细菌,而两者次生共生细菌的组成存在差异。一种肠杆菌科次生共生细菌仅在B型烟粉虱中发现,而另两种次生共生细菌Wolbachia和杀雄菌Arsenophonus仅在非B型中发现。初生共生细菌的系统发育分析表明,B型是入侵生物型,而浙江非B型是本地生物型。

关键词: 烟粉虱, 共生细菌, Wolbachia, 杀雄菌, 系统发育, 16S rDNA, 23S rDNA

Abstract:

Whiteflies are plant sap-feeding insects that harbor prokaryotic symbionts, including primary and secondary endosymbionts. In this study, PCR-based detection of prokaryotic endosymbionts of a Bemisia tabaci B biotype and a non-B biotype China-ZHJ-1 population in Zhejiang, China was performed. Both B and non-B biotype populations harbored a common primary endosymbiont, but different secondary endosymbionts. A secondary endosymbiont of the family Enterobacteriaceae was detected in the B biotype population but not in the non-B biotype. Two other secondary endosymbionts, Wolbachia and Arsenophonus, were detected in non-B biotype population but not in B biotype. Phylogenetic analysis of primary endosymbionts based on 16S rDNA indicates that the non-B biotype is native to China while the B biotype is an invasive biotype.

Key words: Bemisia tabaci, endosymbiont, Wolbachia, Arsenophonus, phylogenesis, 16S rDNA, 23S rDNA