关键词: 褐飞虱, 水稻, 品种, 根系, 叶片, 玉米素核苷, 含量, 酶联免疫吸附法

Abstract:

The response of zeatin ribosides (ZRs) in roots and leaves at different developmental stages of two representative rice varieties to N. lugens nymph infestation was studied using enzyme-linked immunosorbent assay (ELISA). The results indicated that ZR content at the heading stage of Zhendao 2 was more sensitive to N. lugens infestation compared with that at the grain filling stage, with significant reduction of ZR contents in roots and leaves at 3, 6 and 9 days after 15, 30, 60 and 120 nymphs/plant infestations compared with the control. For the grain-filling stage of Zhendao 2, N. lugens infestation did not result in significant reduction of ZR content in roots and leaves except ZR contents in leaves at 9 days after 15, 30, 60, 120 nymphs/plant infestation and ZR content in roots at 9 days after 120 nymph/plant infestations. However, the response of ZR in Xieyou 63 to N. lugens stress was different from Zhendao 2: ZR contents in leaves at 3 days after 15, 30 and 60 nymph infestation increased significantly at the heading stage, and there were no significant alterations of ZR content at the grain filling except with significant increase of ZR contents in leaves at 6 days after 30, 60, 120 nymphs/plant infestation and at 9 days after 120 nymphs/plant infestation. The results demonstrated that the effect of N. lugens infestation on ZR content varied with rice variety and developmental stage.

Key words: Nilaparvata lugens, rice, variety, roots, leaves, zeatin riboside, content, ELISA