›› 2007, Vol. 50 ›› Issue (5): 441-447.

• 研究论文 • 上一篇    下一篇

花胫绿纹蝗和疣蝗配子发生时原癌基因c-kit特异性表达比较研究

赵卓,张敏,吕淑敏,奚耕思*   

  1. (陕西师范大学生命科学学院,西安 710062)
  • 出版日期:2007-06-08 发布日期:2007-10-20
  • 通讯作者: 奚耕思

Comparison of c-kit special expression in gametogenesis of Aiolopus tamulus (Fabricius) and Trilophidia annulata (Thunberg) (Orthoptera: Oedipodidae)

ZHAO Zhuo, ZHANG Min, LU Shu-Min, XI Geng-Si   

  1. (College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China)
  • Online:2007-06-08 Published:2007-10-20
  • Contact: XI Geng-Si

摘要:

应用免疫组织化学和生物统计分析相结合的方法,对花胫绿纹蝗Aiolopus tamulus (Fabricius)和疣蝗Trilophidia annulata (Thunberg)配子发生过程中原癌基因c-kit特异表达进行了比较研究。结果表明:(1)精子发生过程中,精原细胞、初级精母细胞、次级精母细胞和成熟精子中均有不同程度的c-kit蛋白表达,精巢末端还有较粗大的阳性颗粒分布;(2)卵子发生过程中,第1~6阶段卵母细胞中有不同程度的c-kit蛋白特异性表达,但随着卵黄发生的开始逐渐消失;(3)2种蝗虫配子发生过程中c-kit表达存在种间差异。

关键词: 花胫绿纹蝗, 疣蝗, 配子发生, c-kit蛋白, 特异性表达

Abstract: The special expression of c-kit protein in gametogenesis of Aiolopus tamulus (Fabricius) and Trilophidia annulata (Thunberg) collected from Siping, Jilin, Northeast China was studied by using immunohistochemistry and biostatistics. The results showed that: (1) In spermatogenesis, there were different expression levels of c-kit protein in spermatogonia, primary spermatocytes, second spermatocytes and mature sperms, and the biggest positive granules were found at the end of spermary; (2) In oogenesis, there were different expression levels of c-kit protein from the 1st to 6th stage of oocytes, and the positive granules gradually disappeared in vitellogenesis; (3) The expression of c-kit protein in gametogenesis was significantly different between the two species.

Key words: Aiolopus tamulus, Trilophidia annulata, gametogenesis, c-kit protein, special expression