关键词: 实蝇科, 近缘种, 基因芯片, 分类鉴定

Abstract: The present study eatablished genechip technique for Ceratitis capitata, C. rosa and C. cosyra, in which the mitochondrial cytochrome oxidase Ⅰ (COⅠ) gene was used as molecular marker, and the DNA sequences of fruit flies (Diptera: Tephritidae) were used as target sequence. The genechip detection system for fruit flies was composed of detecting probes and quality-control probes for C. capitata. The detecting probes included one family probe, one genus probe, three related species probes and four species specific-probes, and the quality-control probes included anchor probe, positive probe, negative probe and blank probe, with one for each. The results of gene chip showed that the specificity of detecting probes was specific to distinguish the three Ceratitis fruit flies. The detection method showed good repeatability and stability. The results of different stages (egg, pupa, larva, adult) and different geographical provenances of C. capitata were completely consistent. The genechip technique for Ceratitis fruit flies provided rapid detection and species identification method to quarantine fruit flies from imported fresh fruits and vegetables in China. Meanwhile, the technique also could be used in detecting other tephritid and related pests in plant quarantine.

Key words: Tephritidae, Ceratitis capitata, related species, genechip, taxonomic identification