洋虫成虫脱脂蛋白酶解液的抗氧化作用

›› 2008, Vol. 51 ›› Issue (8): 804-809.

洋虫成虫脱脂蛋白酶解液的抗氧化作用

严善春，初众

出版日期:2010-07-27 发布日期:2008-08-20
通讯作者: 严善春

Antioxidant effects of delipidized protein hydrolysate from Martianus dermestoides Chevrolat (Coleoptera, Tenebrionidae) beetles

YAN Shan-Chun

Online:2010-07-27 Published:2008-08-20

摘要/Abstract

摘要： 对洋虫Martianus dermestoides Chevrolat成虫脱脂蛋白酶解液的抗氧化作用进行了研究。用木瓜蛋白酶水解洋虫成虫脱脂蛋白，用截留分子量分别为10 kDa和6 kDa的超滤膜将其分离成3个组分，用邻苯三酚鲁米诺发光法、DPPH自由基清除法、Fenton法和Oyaizu法，分别测定不同分子量的洋虫成虫脱脂蛋白酶解液清除超氧阴离子自由基、DPPH自由基及羟基自由基的能力。结果表明：洋虫成虫蛋白酶解液3个组分都有很好的清除自由基能力，且还原性较强，以分子量＜6 kDa的组分清除效果最佳(P＜0.05)。结果揭示洋虫成虫脱脂蛋白酶解液具有很好的抗氧化作用。

关键词: 洋虫, 脱脂蛋白酶解液, 抗氧化, 天然抗氧化剂, 自由基

Abstract: The antioxidant effects of delipidized protein hydrolysate from Martianus dermestoides Chevrolat beetles were studied. The delipidized proteins from the beetles were hydrolyzed by papain and then separated into three parts using two kinds of ultrafiltration membranes, which permit compounds with the molecular weights of 10 kDa and 6 kDa to pass through separately. The antioxidant effects of the three parts to free radicals O₂^-., DPPH· and ·OH were assayed by the methods of pyrogallol\|luminol, scavenging DPPH, Fenton and Oyaizu, respectively. The results showed that all the three parts of delipidized protein hydrolysates from the beetles had distinct scavenging effects, and the strongest effects were found in the hydrolysate with the molecular weight less than 6 kDa (P＜0.05). We so concluded that the delipidized protein hydrolysate from the beetle has good antioxidant effects.

Key words: Martianus dermestoides, delipidized protein hydrolysate, antioxidant effect, natural antioxidant, free radicals

严善春，初众. 洋虫成虫脱脂蛋白酶解液的抗氧化作用[J]. , 2008, 51(8): 804-809.

YAN Shan-Chun. Antioxidant effects of delipidized protein hydrolysate from Martianus dermestoides Chevrolat (Coleoptera, Tenebrionidae) beetles[J]. , 2008, 51(8): 804-809.

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