Interruptin B对小菜蛾和亚洲玉米螟幼虫的拒食活性及对斜纹夜蛾卵巢细胞的毒力

摘要/Abstract

摘要：

为了研究Interruptin B的杀虫活性, 初步探讨其杀虫作用机理, 本研究采用叶碟法和叶片浸渍法测定了Interruptin B对小菜蛾Plutella xylostella和亚洲玉米螟Ostrinia furnacalis 3龄幼虫的拒食活性和毒杀活性; 采用注射法和血球计数法研究对斜纹夜蛾Spodoptera litura幼虫血细胞数的影响; 采用四甲基偶氮唑盐［3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide, MTT］法测定对斜纹夜蛾卵巢细胞(SL细胞)的增殖抑制作用; 采用倒置显微镜观察对SL细胞形态、密度和贴壁率的影响。结果显示：处理后48 h, Interruptin B对亚洲玉米螟和小菜蛾3龄幼虫的拒食中浓度(AFC₅₀）分别为108.56 和143.88 μg/mL, 致死中浓度(LC₅₀）分别为346.23和194.32 g/mL; 处理后24 h和48 h, 对SL细胞的抑制中浓度(IC₅₀）分别为13.51和4.11 g/mL。Interruptin B处理SL细胞后12-48 h, 细胞密度随时间的延长而下降, 与对照差异显著(P<0.05）; 处理后3, 6, 12, 24和48 h, SL细胞变形率分别为28.18%, 83.90%, 85.18%, 93.93%和100%, 细胞贴壁率分别为96.59%, 89.90%, 83.18%, 5.06%和1.09% , 处理后6-48 h, SL细胞变形率和贴壁率与对照差异显著(P<0.05）。Interruptin B对小菜蛾和亚洲玉米螟3龄幼虫具有良好的拒食和毒杀活性, 能显著降低斜纹蛾幼虫血细胞数量, 对SL细胞具有良好的增殖抑制作用, 可显著降低SL细胞生长密度, 改变细胞形态, 降低贴壁率, 表明该化合物可通过影响昆虫细胞活力毒杀活体昆虫。

关键词: 小菜蛾, 亚洲玉米螟, Interruptin B, 拒食活性, 杀虫活性, 斜纹夜蛾卵巢细胞

Abstract:

In order to ascertain the insecticidal activity and probe into the insecticidal mechanism of Interruptin B, we studied its biological activity against the 3rd larvae of Plutella xylostella and Ostrinia furnacalis with leaf dipping method and the effect on the number of blood corpuscles in Spodoptera litura larvae by injection and blood cell count, and observed the cell proliferation inhibition of S. litura ovary cells (SL cells) using MTT method. Meanwhile, we observed its effect on the shape, density and the adherence rate of cells by inverted microscope. The results indicated that Interruptin B had the antifeedant and insecticidal activity against the 3rd instar larvae of P. xylostella and O. furnacalis. At 48 h post treatment, the AFC₅₀ values of Interruptin B against the 3rd instar larvae of O. furnacalis and P. xylostella were 108.56 g/mL and 143.88 μg/mL, respectively, while the LC₅₀values were 346.23 μg/mL and 194.32 μg/mL, respectively. This compound also had strong inhibitive activity on the growth of S. litura larvae, its IC₅₀ values on SL cells at 24 and 48 h post treatment were 13.51 μg/mL and 4.11 μg/mL, respectively. Significant decrease of cell density were observed by microscope in the Interruptin B treated SL cells (P<0.05). The deformation rates of SL cells were 28.18%, 83.90%, 85.18%, 93.93% and 100%, and the adherence rates were 96.59%, 89.90%, 83.18%, 5.06 % and 1.09% at 3, 6, 12, 24 and 48 h post treatment, respectively. The deformation rate and adherence rate of SL cells treated with Interruptin B were significantly different from those in the control at 6-48 h post treatment (P<0.05）. The results show that Interruptin B possesses the antifeedant and insecticidal activity against P. xylostella and O. furnacalis, and can decrease the blood cells of the larvae of S. litura and the density of SL cells, and has strong inhibitive activity on the growth of S. litura larvae. In addition, it can inhibit cell proliferation, change the cell shape, and decrease the adherence rate of SL cells. These suggest that the insecticidal activity of Interruptin B is realized by affecting the cell activity of insects.

Key words: Plutella xylostella, Ostrinia furnacalis, Interruptin B, antifeedant activity, insecticidal activity, Spodoptera litura ovary cells (SL cells)

黄素青, 徐汉虹, 童松, 张志祥. Interruptin B对小菜蛾和亚洲玉米螟幼虫的拒食活性及对斜纹夜蛾卵巢细胞的毒力[J]. , 2010, 53(10): 1104-1110.

HUANG Su-qing. Antifeedant activity of Interruptin B against Plutella xylostella and Ostrinia furnacalis and cytotoxicity against Spodoptera litura ovary cells[J]. , 2010, 53(10): 1104-1110.

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Interruptin B对小菜蛾和亚洲玉米螟幼虫的拒食活性及对斜纹夜蛾卵巢细胞的毒力

Antifeedant activity of Interruptin B against Plutella xylostella and Ostrinia furnacalis and cytotoxicity against Spodoptera litura ovary cells

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