关键词: 光肩星天牛, 消化酶, 纤维素酶, 酶活性, 寄主选择

Abstract:

To investigate the relationship between cellulase activity and host selection, Anoplophora glabripennis (Motsch.) larvae from four different host trees (Populus alba var. pyramidalis Bunge, Populus nigra var. thevestina (Dode) Bean, Populus simonii × Populus pyramidalis cv. opera Hsu and Salix matsudana f. lobato-glandulosa Fang et Liu) and adults fed on five different tree species were selected. The enzyme activities of endoglucanase andβ-glucosidase in the intestines of the insects were measured. The results showed that in the larvae, the activity of endoglucanase ranged from 1.36 to 2.71 μmol glucose/(g FW·h), and the activity of β-glucosidase ranged from 2.57 to 4.86 μmol glucose/(g FW·h). In the adults fed on different tree species, the activity of endoglucanase ranged from 4.08 to 9.27 μmol glucose/(g FW·h), and the activity of β-glucosidase ranged from 2.87 to 6.08 μmol glucose/(g FW·h). There are no significant differences in cellulase activities among the larvae from different host trees. However, the tree species which the adults fed upon has great effect on the activity of cellulase in the adults.

Key words: Anoplophora glabripennis, digestive enzyme, cellulase, enzyme activity, host selection