›› 2012, Vol. 55 ›› Issue (4): 493-498.doi:

• 简报 • 上一篇    

屋尘螨成螨形态的扫描电镜观察

赵学影, 刘晓宇, 李玲, 孙新, 刘志刚   

  1. 深圳大学过敏反应与免疫学研究所, 广东深圳 518060
  • 收稿日期:2011-11-22 修回日期:2012-03-23 出版日期:2012-04-20 发布日期:2012-04-20
  • 通讯作者: 孙新, 刘志刚 E-mail:zg195910@126.com; sunxin@bbmc.edu.cn
  • 作者简介:赵学影, 女, 1982年生, 安徽亳州人, 硕士研究生, 主要从事分子寄生虫免疫研究, E-mail: zhxy2020@163.com
  • 基金资助:

    国家“863”计划项目(2006AA02A231); 国家自然科学基金项目(81071388, 30760082); 粤港关键领域重点突破项目(20054982207); 深圳市科技计划项目(200630); 深圳市南山区科技计划项目

Morphological observation on adult Dermatophagoides pteronyssinus (Acari: Pyroglyphidae) with scanning electron microscope

ZHAO Xue-Ying, LIU Xiao-Yu, LI Ling, SUN Xin, LIU Zhi-Gang   

  1. Institute of Allergy and Immunology, Medical College, Shenzhen University, Shenzhen, Guangdong 518060, China
  • Received:2011-11-22 Revised:2012-03-23 Online:2012-04-20 Published:2012-04-20
  • Contact: SUN Xin, LIU Zhi-Gang E-mail:zg195910@126.com; sunxin@bbmc.edu.cn
  • About author:zhxy2020@163.com

摘要: 【目的】利用扫描电子显微镜(scanning electron microscopy, SEM)观察屋尘螨Dermatophagoides pteronyssinus颚体、 躯体、 外生殖器及足等的形态结构。【方法】从床尘、 枕尘中采集屋尘螨, 分离出雌雄成螨, 在体视显微镜下清洗处理活螨后, 用SEM观察其外部形态特征。【结果】SEM照片显示, 屋尘螨螯肢钳状, 须肢扁平; 体表具细密皮纹, 似指纹状, 纹间距小于2 μm。外生殖器位于腹面正中, 雌螨为产卵孔, 雄螨生殖孔具1对叶状生殖盖。肛门呈纵行裂孔, 雄螨具2个肛吸盘。雌螨足跗节末端各具爪垫1个, 雄螨跗节Ⅳ具2个吸盘。【结论】本研究观察结果为尘螨鉴定提供了更多依据。

关键词:  尘螨, 屋尘螨, 成螨, 形态, 扫描电子显微镜

Abstract: 【Aim】 The external morphology including the gnathosoma, idiosoma, external genitalia and legs of Dermatophagoides pteronyssinus was observed by using scanning electron microscopy (SEM). 【Methods】 D. pteronyssinus mites were collected from the dust of beds and pillows, and the living adult mites were isolated using the stereoscope and observed with SEM. 【Results】 SEM photographs showed that the cheliceraeare clamp-like and palps flat. Cuticle is covered with fine striations like fingerprint, with distance between striations less than 2 μm. Oviporus of female genitalia is located at the center of the ventral surface of the idiosoma. The male genitalia has a pair of leaflike genital folds. Anus is a longitudinal hole, with two anal suckers in the male. The end of each tarsus of female has a pulvillus, and the tarsus of leg Ⅳ of the male has two suckers. 【Conclusion】 The observations on D. pteronyssinus provide more evidence for identification of dust mites.

Key words: Dust mite; Dermatophagoides pteronyssinus, adult mite, morphology, scanning electron microscopy (SEM)

中图分类号: 

  • Q965