α-萜品醇熏蒸对大麦虫体内抗氧化酶活性的影响

›› 2013, Vol. 56 ›› Issue (1): 47-53.doi:

α-萜品醇熏蒸对大麦虫体内抗氧化酶活性的影响

田雨浓¹，马伟²，韦庆慧³，罗帅¹，韩旭洋¹，陈旭日¹，邱本军¹，马玲^1,*

(1. 东北林业大学林学院，哈尔滨 150040； 2. 黑龙江中医药大学药学院，哈尔滨 150040；3. 东北林业大学生命科学学院，哈尔滨 150040)

出版日期:2013-01-20 发布日期:2013-01-20

Effects of α-terpineol fumigation on the in vivo antioxidant activity in Zophobas morio (Coleoptera: Tenebrionidae)

TIAN Yu-Nong¹, MA Wei², WEI Qing-Hui³, LUO Shuai¹, HAN Xu-Yang¹, CHEN Xu-Ri¹, QIU Ben-Jun¹, MA Ling1,*

(1. School of Forestry, Northeast Forestry University, Harbin 150040, China; 2. Pharmaceutical College, Heilongjiang University of Chinese Medicine, Harbin 150040, China; 3. College of Life Science, Northeast Forestry University, Harbin 150040, China)

Online:2013-01-20 Published:2013-01-20

摘要/Abstract

摘要： 为研究植物挥发性有机化合物α-萜品醇的杀虫活性及作用机理，本研究采用熏蒸法测定了α-萜品醇对大麦虫Zophobas morio（鞘翅目：拟步行甲科）4龄幼虫的急性毒性，并测定了不同熏蒸时间后幼虫体内超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性。结果表明：熏蒸48 h时， α-萜品醇对大麦虫4龄幼虫的LC₅₀和LC₂₀值分别为69.425 μg/L和59.916 μg/L。α-萜品醇（LC₂₀和LC₅₀）处理的4龄幼虫SOD， POD和CAT活性均表现为先升高后降低的趋势。据此推测， α-萜品醇在幼虫体内积累显著影响幼虫体内SOD， POD和CAT活性，降低虫体内自由基的清除能力，从而对其产生毒害作用。

关键词: 大麦虫； α-萜品醇；熏蒸；急性毒性；保护酶, 酶活性

Abstract: In order to study the insecticidal activity and toxicity mechanism of α-terpineol, a plant volatile organic compound, we tested the acute toxicity of α-terpineol against the 4th instar larvae of Zophobas morio in a gas-fumigation system and investigated the in vivo activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the treated larvae at different fumigation duration. The results showed that the LC₅₀ and LC₂₀ values of α-terpineol against the 4th instar larvae in acute toxicity test at 48 h after fumigation were 69.425 μg/L and 59.916 μg/L, respectively. Under the fumigation of α-terpineol at two concentrations (LC₅₀ and LC₂₀ ), the activities of SOD, POD and CAT showed a time-course change, first increasing and then decreasing. It is so inferred that the gradual accumulation of α-terpineol in the treated larvae produces significantly negative effects on the activities of SOD, POD and CAT in their bodies, and this may lead to a decline in scavenging activity of free radicals and stronger toxic effects on Z. morio larvae.

Key words: Zophobas morio, α-terpineol, fumigation, acute toxicity, protective enzymes, enzyme activity

田雨浓, 马伟, 韦庆慧, 罗帅, 韩旭洋, 陈旭日, 邱本军, 马玲. α-萜品醇熏蒸对大麦虫体内抗氧化酶活性的影响[J]. , 2013, 56(1): 47-53.

TIAN Yu-Nong, MA Wei, WEI Qing-Hui, LUO Shuai, HAN Xu-Yang, CHEN Xu-Ri, QIU Ben-Jun, MA Ling. Effects of α-terpineol fumigation on the in vivo antioxidant activity in Zophobas morio (Coleoptera: Tenebrionidae)[J]. , 2013, 56(1): 47-53.

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