幼虫密度对草地螟血细胞数量和组成的影响

›› 2013, Vol. 56 ›› Issue (6): 630-637.

幼虫密度对草地螟血细胞数量和组成的影响

杨志兰^1,2，程云霞¹，罗礼智^1,*，孔海龙³，张蕾¹，雷朝亮²

（1. 中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京 100193; 2. 华中农业大学植物科学技术学院，
湖北省昆虫资源利用与害虫可持续治理重点实验室，武汉 430070; 3. 扬州大学园艺与植物保护学院，江苏扬州 225009）

出版日期:2013-06-20 发布日期:2013-06-20

Effects of larval density on the number and composition of hemocytes in the beet webworm, Loxostege sticticalis (Lepidoptera: Pyralidae)

YANG Zhi-Lan^1,2, CHENG Yun-Xia¹, LUO Li-Zhi¹,*, KONG Hai-Long³, ZHANG Lei¹, LEI Chao-Liang²

(1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China ; 2. Hubei Insect Resource Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 3. College of Plant Protection and Horticulture, Yangzhou University, Yangzhou, Jiangsu 225009, China)

Online:2013-06-20 Published:2013-06-20

摘要/Abstract

摘要： 为了阐明幼虫密度对草地螟Loxostege sticticalis L.（鳞翅目：螟蛾科）细胞免疫能力的影响，本研究调查了在活体灰菜植株上1，5，10和20头/瓶（900 mL）4种密度条件下的其5龄幼虫血细胞种类、数量和组成。结果表明：草地螟幼虫血淋巴中有原血细胞、浆血细胞、颗粒血细胞、珠血细胞和类绛色血细胞等5种（类）血细胞。血细胞总数、浆血细胞、颗粒血细胞数量随幼虫密度的增加而显著递增，但原血细胞、珠血细胞和类绛色血细胞数量在幼虫密度间的差异不明显；各种血细胞所占血细胞总数的比例在4个密度中的排序相同，但10和20头/瓶密度下的浆血细胞比例显著高于1头/瓶的，其余4种血细胞的比例在不同密度之间无显著差异。可见，幼虫密度主要是通过影响草地螟幼虫浆血细胞和颗粒血细胞的数量及血细胞总数，从而影响草地螟的细胞免疫能力。

关键词: 草地螟, 幼虫密度, 血细胞, 细胞免疫, 浆血细胞, 颗粒血细胞

Abstract: To understand the roles of larval density in the cellular immunity of the beet webworm, Loxostege sticticalis L. (Lepidoptera: Pyralidae), the types, number and composition of hemocytes in its 5th instar larvae reared on intact plants of Chenopodium album L. at the density of 1, 5, 10, and 20 larvae/jar (900 mL) were investigated. Five types of hemocytes were identified from larval L. sticticalis, i.e., prohemocytes, granular hemocytes, plasmatocytes, spherulocytes and oenocytoids. The numbers of plasmatocytes, granular hemocytes and total hemocytes increased as the larval density increased, but the numbers of prohemocytes, spherulocytes, and oenocytoids were insignificantly affected by the larval density. The proportion of each hemocyte type to the total haemocetes ranked in a similar order in the four treatments but the ratios of plasmatocytes at the density of 10 and 20 larvae/jar were remarkably higher than that of the isolated larvae. These results suggest that the larval density can affect the number of plasmatocytes and granular hemocytes, and this impact in turn can affect the cellular immune capacity in larval L. sticticalis.

Key words: Loxostege sticticalis, larval density, hemocytes, cellular immunity, plasmatocytes, granular hemocytes

杨志兰, 程云霞, 罗礼智, 孔海龙, 张蕾, 雷朝亮. 幼虫密度对草地螟血细胞数量和组成的影响[J]. , 2013, 56(6): 630-637.

YANG Zhi-Lan, CHENG Yun-Xia, LUO Li-Zhi, KONG Hai-Long, ZHANG Lei, LEI Chao-Liang. Effects of larval density on the number and composition of hemocytes in the beet webworm, Loxostege sticticalis (Lepidoptera: Pyralidae)[J]. , 2013, 56(6): 630-637.

[1]	呼晓庆, 杨兆富. 基于线粒体COI、Cytb和COII基因的中国草地螟不同地理种群遗传分化分析[J]. 昆虫学报, 2019, 62(6): 720-733.
[2]	阿孜古丽·阿布力孜, 牙森·沙力, 族米娜, 阿曼古力·吐尼亚孜, 吴乐年, 徐光青, 赵莉. 新疆阿勒泰地区草地螟成虫发生特征[J]. , 2018, 61(9): 1106-1113.
[3]	倪若尧, 孟茜, 张寰, 张继红, 秦启联. 小金蝠蛾幼虫血细胞的种类、形态及细胞免疫功能[J]. , 2018, 61(4): 432-438.
[4]	王远, 张若男, 陈雪, 顾偌铖, 苏睿, 钟仰进, 杨婉莹. 20-羟基蜕皮酮调控昆虫先天免疫的分子机制研究进展[J]. 昆虫学报, 2018, 61(11): 1328-1339.
[5]	辛蓓, 张少斌, 刘佩旋, 郑雅楠. 白蛾周氏啮小蜂毒液对美国白蛾蛹血细胞免疫的影响[J]. 昆虫学报, 2016, 59(7): 699-706.
[6]	陈晓, 姜玉英, 孟正平, 陈阔, 康爱国, 李春民, 翟保平. 极端气候成为我国草地螟暴发周期终结的重要因子[J]. 昆虫学报, 2016, 59(12): 1363-1375.
[7]	王斯奇, 陈俊, 章娟娟, 杨之帆. AChE在水稻抗性诱导的褐飞虱凋亡中肠细胞中的定位及表达[J]. 昆虫学报, 2016, 59(10): 1033-1042.
[8]	罗剑峰, 刘欢, 郭子俊, 王国才, 凌冰. 苦瓜素Ⅰ和苦瓜苷B对亚洲玉米螟Ofh细胞的增殖抑制和致坏死作用[J]. 昆虫学报, 2016, 59(10): 1093-1102.
[9]	童应华，李万里，马淑娟. 金龟子绿僵菌及其粗毒素对樟巢螟幼虫的致病性[J]. , 2014, 57(4): 418-427.
[10]	徐忠宝, 刘爱萍，徐林波，高书晶，王建梅，苏春芳，康爱囯，张玉慧. 草地螟阿格姬蜂的滞育诱导和滞育茧的低温贮藏[J]. , 2013, 56(10): 1160-1165.
[11]	孔海龙, 罗礼智, 江幸福, 张蕾, 杨志兰, 胡毅. 幼虫密度对草地螟食物利用率及消化酶活性的影响[J]. , 2012, 55(3): 361-366.
[12]	张健华, 罗礼智, 江幸福, 张蕾 . 草地螟滞育幼虫的蛋白和核酸含量变化[J]. , 2012, 55(2): 156-161.
[13]	陈阳, 姜玉英, 刘家骧, 吕英, 孟正平, 陈静, 唐继洪. 标记回收法确认我国北方地区草地螟的迁飞[J]. , 2012, 55(2): 176-182.
[14]	张明明, 初源, 赵章武, 安春菊. 昆虫天然免疫反应分子机制研究进展[J]. , 2012, 55(10): 1221-1229.
[15]	黄芳, 时敏, 陈学新, 章金明. 半闭弯尾姬蜂寄生及其毒液对小菜蛾幼虫血细胞吞噬作用的影响[J]. , 2011, 54(9): 989-996.