灰飞虱唾液腺三大解毒酶家族的转录组分析

›› 2013, Vol. 56 ›› Issue (12): 1509-1515.

灰飞虱唾液腺三大解毒酶家族的转录组分析

刘长莉^1,#，卢利霞^1,2,#，许艳丽^1,3，杨鹏程⁴，崔峰^2,*

(1. 东北林业大学生命科学学院，哈尔滨 150040; 2. 中国科学院动物研究所，农业虫害鼠害综合治理研究国家重点实验室，北京 100101;
3. 中国科学院东北地理与农业生态研究所, 哈尔滨 150081; 4. 中国科学院北京生命科学研究院，北京 100101)

出版日期:2013-12-20 发布日期:2013-12-20

Transcriptomic analysis of three detoxification enzyme families in the salivary glands of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae)

LIU Chang-Li^1,#, LU Li-Xia^1,2,#, XU Yan-Li^1,3, YANG Peng-Cheng⁴, CUI Feng^2,*

(1. College of Life Science, Northeast Forestry University, Harbin 150040, China; 2. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; 3. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China; 4. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China)

Online:2013-12-20 Published:2013-12-20

摘要/Abstract

摘要： 灰飞虱Laodelphax striatellus （Fallén）是危害多种禾本科经济作物的重要刺吸式害虫。唾液腺对刺吸式口器昆虫取食植物尤其重要，其分泌的唾液可以帮助刺吸式口器昆虫刺穿植物、消化食物、解毒植物的次生物质。细胞色素P450单加氧酶（cytochrome P450 monooxygenase, P450）、谷胱甘肽S转移酶（glutathione Stransferase, GST）和羧酸酯酶（carboxylesterase, CarE）是昆虫主要的解毒酶系。为了分析解毒酶基因在灰飞虱唾液腺中的表达谱，本研究对灰飞虱成虫唾液腺进行转录组测序、重头组装和注释，并与豌豆蚜Acyrthosiphon pisum和西方蜜蜂Apis mellifera的同源蛋白进行系统发育分析。发现有9个谷胱甘肽S转移酶（glutathione Stransferase, GST）基因、 22个羧酸酯酶（carboxylesterase, CarE）基因和39个细胞色素P450单加氧酶（cytochrome P450 monooxygenase, P450）基因在灰飞虱唾液腺中表达。通过对同源蛋白进行系统发育分析，发现灰飞虱唾液腺大部分的CarE是参与消化/解毒和激素/信息素的加工，而参与神经/发育的CarE很少；灰飞虱唾液腺表达的P450基因远远少于豌豆蚜和西方蜜蜂基因组的P450基因数，且只有CYP6和CYP4家族的成员； GST家族在3种昆虫的保守性最高。研究结果为灰飞虱对寄主植物和杀虫剂的适应性研究奠定了基础。

关键词: 灰飞虱, 唾液腺, 谷胱甘肽S转移酶, 羧酸酯酶, 细胞色素P450, 系统发育

Abstract: The small brown planthopper, Laodelphax striatellus (Fallén), is an important piercing-sucking pest of many economic Poaceae plants. Salivary glands play a key role in plant feeding for piercing-sucking pests because the secreted saliva help them pierce and digest plants and detoxify secondary metabolites. The detoxification system of insects is mainly composed of cytochrome P450 monooxygenase (P450), glutathione S-transferase (GST), and carboxylesterase (CarE). In order to clarify the expression profiles of detoxification enzyme genes in salivary glands of Laodelphax striatellus, the transcriptome of adult salivary glands was sequenced, de novo assembled and annotated, and phylogenetic analysis of the three detoxification enzyme families among L. striatellus, Acyrthosiphon pisum, and Apis mellifera was conducted in this study. The results showed that 9 glutathione S-transferases (GST) genes, 22 carboxylesterases (CarE) genes, and 39 cytochrome P450 monooxygenases (P450) genes were found expressed in salivary glands. Phylogenetic analysis showed that most of CarEs take part in dietary/detoxification and hormone/semiochemical processing while few CarEs are related to neuro/development in L. striatellus salivary glands. The number of P450 genes expressed in L. striatellus salivary glands is fewer than that in the genomes of A. pisum and A. mellifera, and only CYP6 and CYP4 genes were expressed in L. striatellus salivary glands. GSTs were the most conserved in the three insects. These results underpin the researches of adaptation to host plants and insecticides of L. striatellus.

Key words: Laodelphax striatellus, salivary gland, glutathione S-transferase, carboxylesterase, cytochrome P450 monooxygenase, phylogeny

刘长莉，卢利霞，许艳丽，杨鹏程，崔峰. 灰飞虱唾液腺三大解毒酶家族的转录组分析[J]. , 2013, 56(12): 1509-1515.

LIU Chang-Li, LU Li-Xia, XU Yan-Li, YANG Peng-Cheng, CUI Feng. Transcriptomic analysis of three detoxification enzyme families in the salivary glands of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae)[J]. , 2013, 56(12): 1509-1515.

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