基于微卫星标记的印度中部 Madhya Pradesh 地区疟疾强化控制和非强化控制区的斯氏按蚊基因流和种群遗传结构分析（英文）

›› 2015, Vol. 58 ›› Issue (1): 74-81.

基于微卫星标记的印度中部 Madhya Pradesh 地区疟疾强化控制和非强化控制区的斯氏按蚊基因流和种群遗传结构分析（英文）

Arvind SHARMA¹, Richa SHARMA¹, Ashwani KUMAR¹, Madhulika DUBE², Surendra Kumar GAKHAR^1,*

(1. Centre for Biotechnology, Maharshi Dayanand University, Rohtak124001, India; 2. Department of Statistics, Maharshi Dayanand University, Rohtak124001, India)

出版日期:2015-01-20 发布日期:2015-01-20

Gene flow and population genetic structure of Anopheles stephensi (Diptera: Culicidae) in the areas under Enhanced Malaria Control Project (EMCP) and non-EMCP of Madhya Pradesh, Central India using microsatellite markers(In English)

Arvind SHARMA¹, Richa SHARMA¹, Ashwani KUMAR¹, Madhulika DUBE², Surendra Kumar GAKHAR^1,*

(1. Centre for Biotechnology, Maharshi Dayanand University, Rohtak124001, India; 2. Department of Statistics, Maharshi Dayanand University, Rohtak124001, India)

Online:2015-01-20 Published:2015-01-20
Contact: Corresponding author, Tel.: +919896014951; E-mail:surengak@gmail.com

摘要/Abstract

摘要： 【目的】斯氏按蚊 Anopheles stephensi 是亚洲东南部城市人体疟疾的主要媒介，印度12%的疟疾病例由其引起。本实验研究了印度中部Madhya Pradesh 地区东北部的疟疾强化控制（EMCP）区和非强化控制（非EMCP）区斯氏按蚊的基因流。在EMCP区，由于采用了各种疟疾防控措施因而疟疾病例首先降低，但是很快回升，说明总的疟疾风险维持稳定。【方法】应用7个微卫星位点，对印度中部Madhya Pradesh 地区东北部的4个EMCP区和非EMCP区采集的斯氏按蚊进行基因分型，以分析各种群参数。【结果】发现各标记在所有种群中表现出高度的多态性。在两区间未发现很大的遗传多样性。观察到EMCP区的东部种群(F_ST=0.0485, R_ST=0.1112)比非EMCP区的北部种群(F_ST=0.020, R_ST=0.0145)具有较高的遗传分化，在EMCP区和非EMCP区之间观察到较高的基因流（12.90, 6.16, 5.06 和2.38）。R_ST的灵敏度高于 F_ST，说明分化可能是由于突变而非遗传漂变引起的。【结论】本研究表明，在EMCP区和非EMCP区内以及EMCP区和非EMCP区之间存在很高的基因流。基因流水平高以及抗虫性的发展似乎是EMCP区和非EMCP区疟疾病例发生增加的重要原因。

关键词: 斯氏按蚊: 微卫星标记, 基因流, 遗传分化, 遗传漂变, 疟疾

Abstract: 【Aim】Anopheles stephensi is a major urban human malaria vector in South-East Asia contributing about twelve percent of malaria cases in India. The gene flow in An. stephensi belonging to the areas under Enhanced Malaria Control Project (EMCP) and non-EMCP of north-eastern regions of Madhya Pradesh (central India) was studied as initially there was a decline in malaria cases in EMCP areas due to various malaria control strategies but malaria appeared to be back-track with malaria cases increasing readily, indicating that the overall malaria risk remained stable. 【Methods】 The An. stephensi mosquitoes collected from four different areas under EMCP and non-EMCP from north eastern Madhya Pradesh (central India) were genotyped using 7 microsatellite loci for analysis of various population parameters. 【Results】 The markers were found to be highly polymorphic in all the populations. Not much genetic diversity was found between the two regions. Higher genetic differentiation was observed in eastern populations under EMCP (F_ST= 0.0485, R_ST=0.1112) than northern populations under non-EMCP (F_ST=0.020, R_ST=0.0145) and high gene flow (12.90, 6.16, 5.06 and 2.38) was observed between EMCP and non-EMCP areas. The higher sensitivity of R_ST than F_ST indicated that the differentiation was probably caused by the mutation but not due to genetic drift. 【Conclusion】 The study revealed substantially high gene flow within and between EMCP and non-EMCP areas. The high gene flow combined with development of insecticide resistance seems to be a potent reason for increase in malaria cases in EMCP as well as non-EMCP areas.

Key words: Anopheles stephensi, microsatellite markers, gene flow, genetic differentiation, genetic drift, malaria

Arvind SHARMA, Richa SHARMA, Ashwani KUMAR, Madhulika DUBE, Surendra Kumar GAKHA. 基于微卫星标记的印度中部 Madhya Pradesh 地区疟疾强化控制和非强化控制区的斯氏按蚊基因流和种群遗传结构分析（英文）[J]. , 2015, 58(1): 74-81.

Arvind SHARMA, Richa SHARMA, Ashwani KUMAR, Madhulika DUBE, Surendra Kumar GAKHAR. Gene flow and population genetic structure of Anopheles stephensi (Diptera: Culicidae) in the areas under Enhanced Malaria Control Project (EMCP) and non-EMCP of Madhya Pradesh, Central India using microsatellite markers(In English)[J]. , 2015, 58(1): 74-81.

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