关键词: 大垫尖翅蝗, 转录组, 基因注释, GO数据库, COG数据库, KEGG数据库, 简单重复序列

Abstract: 【Aim】 Epacromius coerulipes (Ivanov) is one of the most widely distributed grassland locusts. However, there is a lack of genomic resources. In order to obtain genetic data of E. coerulipes, this study conducted the transcriptome sequencing and analysis. 【Methods】 De novo assembly of the transcriptome was conducted using Illumina paired-end sequencing technology. 【Results】 In total, 63 033 unigenes with a mean length of 772 bp and an N50 length of 1 589 bp were obtained. Of these unigenes, 25 132(39.87%) unigenes were matched with the known proteins in the National Center for Biotechnology Information (NCBI) database, as determined by BLAST search, with 24 841, 16 490, 11 558 and 8 013 unigenes assigned to Nr database, Swiss-Prot, Gene Ontology (GO) and Clusters of Orthologous Groups (COG), respectively. In KEGG database, a total of 7 218 unigenes were assigned to 218 known pathways. Among these, 189 unigenes were involved in metabolic pathways of xenobiotics or drugs. In-depth analysis of the data showed that 213 unigenes were identified as potentially involved in the detoxification of xenobiotics, and 29 unigenes were identified as encoding insecticide target proteins. In addition, 5 696 simple sequence repeats (SSRs) were detected. 【Conclusion】 This study lays the foundation for further research on gene function analysis and molecular resistance mechanisms to insecticides in E. coerulipes.

Key words: Epacromius coerulipes, transcriptome, gene annotation, Gene Ontology (GO), Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG), simple sequence repeat (SSR)