麦红吸浆虫幼虫不同滞育时期体内山梨醇含量及山梨醇脱氢酶基因表达水平变化

doi:10.16380/j.kcxb.2016.02.001

昆虫学报 ›› 2016, Vol. 59 ›› Issue (2): 119-126.doi: 10.16380/j.kcxb.2016.02.001

• 研究论文 • 下一篇

麦红吸浆虫幼虫不同滞育时期体内山梨醇含量及山梨醇脱氢酶基因表达水平变化

刘洋¹, 高贺¹, 李雪娇¹, 仵均祥¹, 成卫宁^1,*, 朱克岩²

（1. 西北农林科技大学, 农业部西北黄土高原作物有害生物综合治理重点实验室, 陕西杨凌 712100; 2. Department of Entomology, Texas A&M University, Texas 77843, USA）

出版日期:2016-02-20 发布日期:2016-02-20
作者简介:刘洋, 女, 1992年6月生, 河南三门峡人, 硕士研究生, 研究方向为害虫综合治理, E-mail: liuyang5820718@sina.cn

Changes in sorbitol content and the expression level of sorbitol dehydrogenase gene in Sitodiplosis mosellana (Diptera: Cecidomyiidae) larvae at different diapause stages

LIU Yang¹, GAO He¹, LI Xue-Jiao¹, WU Jun-Xiang¹, CHENG Wei-Ning^1,*, ZHU-SALZMAN Ke-Yan²

(1. Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. Department of Entomology,Texas A&M University, Texas 77843, USA)

Online:2016-02-20 Published:2016-02-20

摘要/Abstract

摘要： 【目的】本研究旨在明确麦红吸浆虫 Sitodiplosis mosellana (Géhin)滞育进程中山梨醇含量及山梨醇脱氢酶基因（SmSDH）mRNA表达水平的变化规律，探讨麦红吸浆虫滞育与山梨醇含量及 SmSDH 表达水平的关系。【方法】分别采用比色法和实时荧光定量PCR方法，对2013年5月-2014年3月不同时间陕西杨凌养虫圃内麦红吸浆虫滞育前后及滞育期幼虫体内山梨醇含量及 SmSDH2a 和 SmSDH2b mRNA表达水平进行分析。【结果】麦红吸浆虫幼虫滞育前山梨醇含量最高（为9.41 μg/mg）；滞育期间，12月份含量最高(为8.50 μg/mg)；滞育解除后山梨醇含量为3.25~3.49 μg/mg，显著低于滞育前和整个滞育期（P＜0.05）。山梨醇脱氢酶基因 SmSDH2a 及SmSDH2b 在滞育进程中mRNA表达水平变化趋势相同，且与滞育强度递减趋势一致，即进入滞育后表达水平显著降低（P＜0.05）并一直稳定在较低水平，滞育解除后逐渐升高并达到最高。滞育期间，同期不同滞育状态幼虫之间山梨醇含量和 SmSDH2a 表达水平差异不明显，但裸露幼虫 SmSDH2b 表达水平始终高于结茧幼虫。【结论】山梨醇和 SmSDH 参与麦红吸浆虫滞育调控，与滞育维持和解除密切相关。

关键词: 麦红吸浆虫, 滞育, 山梨醇, 山梨醇脱氢酶, 基因表达

Abstract: 【Aim】 This study aims to ascertain the change patterns of sorbitol content and the mRNA expression level of sorbitol dehydrogenase gene ( SmSDH) in Sitodiplosis mosellana(Géhin) larvae at different diapause stages and to explore the relationship between diapause and sorbitol content and the expression level of SmSDH in S. mosellana. 【Methods】 Colorimetry and real-time quantitative PCR were respectively used to analyze the sorbitol content and mRNA transcription level of SmSDH2aand SmSDH2b in pre-diapause, diapause and post-diapause larvae of S. mosellana collected from a field insectary in Yangling, Shaanxi during May 2013 to March 2014. 【Results】 The sorbitol content in pre-diapause larvaeof S. mosellana was the highest (9.41 μg/mg). During diapause, the sorbitol content in December was the highest, which was 8.50 μg/mg. The sorbitol content in post-diapause larvae was 3.25-3.49 μg/mg, significantly lower than those in pre-diapause and diapause larvae (P<0.05). Change trends of the mRNA transcription levels of SmSDH2a and SmSDH2b in the process of diapause were similar and closely consistent with the decreasing trend of diapause intensity. Namely, the relative expression levels of SmSDH2a and SmSDH2b reduced significantly after the larvae entered diapause (P<0.05) and maintained at a low level during diapause. The relative expression levels of SmSDH2a and SmSDH2b gradually increased and reached the highest after diapause termination. No obvious difference was found in sorbitol content and the relative expression level of SmSDH2a between no-cocooned and cocooned larvae, but the expression level of SmSDH2b in no-cocooned larvae was higher than that in cocooned larvae at the same diapause stage. 【Conclusion】 Sorbitol and SmSDH are involved in diapause ofS. mosellana and play important roles in diapause maintenance and termination.

Key words: Sitodiplosis mosellana, diapause, sorbitol, sorbitol dehydrogenase, gene expression

刘洋, 高贺, 李雪娇, 仵均祥, 成卫宁, 朱克岩. 麦红吸浆虫幼虫不同滞育时期体内山梨醇含量及山梨醇脱氢酶基因表达水平变化[J]. 昆虫学报, 2016, 59(2): 119-126.

LIU Yang, GAO He, LI Xue-Jiao, WU Jun-Xiang, CHENG Wei-Ning, ZHU-SALZMAN Ke-Yan. Changes in sorbitol content and the expression level of sorbitol dehydrogenase gene in Sitodiplosis mosellana (Diptera: Cecidomyiidae) larvae at different diapause stages[J]. Acta Entomologica Sinica, 2016, 59(2): 119-126.

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麦红吸浆虫幼虫不同滞育时期体内山梨醇含量及山梨醇脱氢酶基因表达水平变化

Changes in sorbitol content and the expression level of sorbitol dehydrogenase gene in Sitodiplosis mosellana (Diptera: Cecidomyiidae) larvae at different diapause stages

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