昆虫学报 ›› 2016, Vol. 59 ›› Issue (4): 456-463.doi: 10.16380/j.kcxb.2016.04.011

• 研究论文 • 上一篇    下一篇

小菜蛾幼虫肠道细菌桃色欧文氏菌的代谢表型组学分析(英文)

李文红1, 2, 金道超1,*, 李凤良2, 金剑雪2, 程英2   

  1. (1. 贵州大学昆虫研究所, 贵阳 550025; 2. 贵州省植物保护研究所, 贵阳 550006)
  • 出版日期:2016-04-20 发布日期:2016-04-20

Phenotypic fingerprints of bacterium Erwinia persicina from larval gut of the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) (In English)

LI Wen-Hong1,2, JIN Dao-Chao1,*, LI Feng-Liang2,JIN Jian-Xue2, CHENG Ying2   

  1. (1. Institute of Entomology, Guizhou University, Guiyang 550025, China; 2. Guizhou Institute of Plant Protection, Guiyang 550006, China)
  • Online:2016-04-20 Published:2016-04-20

摘要: 【目的】肠道细菌桃色欧文氏菌Erwinia persicina是小菜蛾Plutella xylostella幼虫肠道的优势细菌,本研究旨在阐明桃色欧文氏菌的代谢表型特征。【方法】采用BIOLOG细胞表型芯片技术系统研究了桃色欧文氏菌的细胞表型;采用PM 1-10代谢板,对桃色欧文氏菌的950种代谢表型进行了测定。【结果】桃色欧文氏菌能代谢39.47%的碳源、89.74%的氮源、100%的硫源和100%的磷源;高效代谢的碳源为有机酸类和碳水化合物类,高效代谢的氮源为氨基酸类。该肠道细菌表现出261种不同的氮源代谢通路和95种生物合成通路。桃色欧文氏菌具有广泛的适应性,能在分别具有高达9%氯化钠、4%氯化钾、5%硫酸钠、20%乙二醇、6%甲酸钠、2%尿素、6%乳酸钠、200 mmol/L 磷酸钠(pH 7.0)、20 mmol/L苯甲酸钠(pH 5.2)、100 mmol/L硫酸铵(pH 8.0)、100 mmol/L硝酸钠和100 mmol/L亚硝酸钠的渗透溶液中正常代谢;其适应pH值范围为4.5~10,最适7.0。在多种氨基酸的作用下,桃色欧文氏菌均表现出脱羧酶和脱氨酶活性。【结论】桃色欧文氏菌的代谢特征增加了我们对该肠道细菌,特别是其与宿主昆虫的互作及其在肠道环境中的适应性的认识,同时表明通过降低桃色欧文氏菌密度来防治小菜蛾的可能性。

关键词:  桃色欧文氏菌, 小菜蛾, 表型芯片, 代谢指纹图谱, 肠道细菌

Abstract: 【Aim】 Gut bacterium Erwinia persicina is one of the dominant bacterial species in the larval gut of the diamondback moth, Plutella xylostella. The aim of the present study is to investigate the phenotypic characteristics of this bacterium. 【Methods】 The cell phenotype of E. persicina was analyzed with BIOLOG Phenotype MicroArray (PM). Totally 950 different metabolic phenotypes were tested using the PM plates 1-10. 【Results】 E. persicina was able to metabolize 39.47% of the tested carbon sources, 89.74% of nitrogen sources, 100% of sulfur sources, and 100% of phosphorus sources. Most informative utilization patterns for carbon sources of E. persicina were organic acids and carbohydrates, and for nitrogen were various amino acids. The bacterium had 261 different nitrogen metabolic pathways and 95 different biosynthetic pathways. It had a wide range of adaptabilities, and could still metabolize in osmolytes with up to 9% sodium chloride, 4% potassium chloride, 5% sodium sulfate, 20% ethylene glycol, 6% sodium formate, 2% urea, 6% sodium lactate, 200 mmol/L sodium phosphate (pH 7.0), 20 mmol/L sodium benzoate (pH 5.2), 100 mmol/L ammonium sulfate (pH 8.0), 100 mmol/L sodium nitrate, and 100 mmol/L sodium nitrite, respectively. It also exhibited active metabolism under pH values between 4.5 and 10, with optimal pH around 7.0. The gut bacterium showed both decarboxylase and deaminase activities in the presence of various amino acids. 【Conclusion】 The phenotypic characterization of E. persicina increased our knowledge of the bacterium, in particular its interactions with insect hosts and the adaptability in gut environments, and showed us some possible approaches to controlling diamondback moth through decreasing E. persicina density.

Key words:  Erwinia persicina, Plutella xylostella, phenotype microarray, metabolic fingerprint, gut bacteria