昆虫学报 ›› 2016, Vol. 59 ›› Issue (11): 1159-1165.doi: 10.16380/j.kcxb.2016.11.002

• 研究论文 • 上一篇    下一篇

增效醚对二化螟幼虫7-乙氧基香豆素-O-脱乙基酶和羧酸酯酶活性的影响

李阳, 史雪岩*, 高希武*   

  1. (中国农业大学植物保护学院昆虫系, 北京100193)
  • 出版日期:2016-11-20 发布日期:2016-11-20

In vivo effects of piperonyl butoxide on the activities of 7-ethoxycoumarin O-deethylase and carboxylesterase in larval Chilo suppressalis (Lepidoptera: Pyralidae)

LI Yang, SHI Xue-Yan*, GAO Xi-Wu*   

  1. (Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China)
  • Online:2016-11-20 Published:2016-11-20

摘要: 【目的】通过研究二化螟Chilo suppressalis细胞色素P450酶活性特征及增效醚(piperonyl butoxide,PBO)对二化螟幼虫7-乙氧基香豆素-O-脱乙基酶(ECOD)和羧酸酯酶(CarE)活性影响的时间效应,为有效进行二化螟的防治及抗药性治理提供基础的研究数据。【方法】采用以7-乙氧基香豆素为底物的荧光比色法,测定了不同龄期二化螟 ECOD活性,并研究了经PBO(0.69 μg/头)点滴处理后24 h内每隔1 h二化螟4龄幼虫ECOD及 CarE活性的变化。【结果】二化螟的ECOD活性从1龄幼虫至蛹期呈现出先升高后降低的变化趋势,其中ECOD活性在4龄幼虫期升至最高值14.11±1.01 pmol/mg pro·min,而在蛹期降到最低值(1.34±0.11 pmol/mg pro·min)。PBO处理对二化螟4龄幼虫ECOD活性的影响具有随时间而变化的抑制-诱导-抑制的动态效应:在处理后4-9 h,PBO对ECOD活性具有抑制作用,其中在处理后6 h时的抑制作用最强,抑制率达到21%;在处理后10-11 h,PBO诱导了ECOD活性升高;在处理后15-17 h,又表现为抑制作用。PBO处理二化螟后10-12 h和18-20 h对二化螟的CarE活性具有诱导作用,而在14-17 h和20-21 h表现为抑制作用。【结论】不同龄期二化螟幼虫及蛹的ECOD活性存在一定差异。PBO点滴处理对二化螟4龄幼虫的ECOD和 CarE活性具有时间依赖的抑制或诱导效应。  

关键词: 二化螟, 增效醚, 细胞色素P450酶, 7-乙氧基香豆素-O-脱乙基酶, 羧酸酯酶

Abstract: 【Aim】 In order to provide data for efficient management of insecticide resistance of Chilo suppressalis (Walker), we measured the cytochrome P450-mediated 7-ethoxycoumarin-O-deethylase (ECOD) activity in different instars of larval C. suppressalis, and evaluated the time-dependent in vivo effects of piperonyl butoxide (PBO) treatment on the activities of ECOD and carboxylesterase (CarE) in the 4th instar larvae. 【Methods】 By using 7-ethoxycoumarin as the substrate, the ECOD activity in different instar larvae and pupae of C. suppressalis was determined by fluorospectrophotometric analysis. Variations of ECOD and CarE activities in the 4th instar larvae were examined at each hour within 24 hours after treatment topically by PBO (0.69 μg/larva). 【Results】 The ECOD activity in C. suppressalis from the 1st instar larval stage to the pupal stage showed a firstly increasing and then decreasing trend. The ECOD activity increased to the highest level of 14.11±1.01 pmol/mg pro·min in the 4th instar larvae, while decreased to the lowest (1.34±0.11 pmol/mg pro·min) in pupae. PBO exhibited time-dependent effects on the ECOD activity of the 4th instar larvae. As compared with the untreated control, PBO treatment inhibited the ECOD activity during 4-9 h with a maximal inhibition rate of 21% at 6 h, induced its activity during 10-11 h, and then inhibited its activity during 15-17 h. In addition, PBO exposure also affected the CarE activity in the 4th instar larvae, showing inhibition effects during 14-17 h and 20-21 h and induction effects during 10-12 h and 18-20 h after treatment. 【Conclusion】 The ECOD activity varies in different instar larvae and pupae of C. suppressalis. PBO treatment displays time-dependent effects of inhibition or induction on the activities of ECOD and CarE in the 4th instar larvae of C. suppressalis.  

Key words: Chilo supperssalis, piperonyl butoxide, cytochrome P450, 7-ethoxycoumarin-O-deethylase (ECOD), carboxylesterase