• 研究论文 •

### 高CO2浓度对两种色型豌豆蚜体内营养物质含量及消化酶活性的影响

1. （1. 甘肃农业大学资源环境学院, 兰州 730070; 2. 甘肃农业大学植物保护学院, 兰州 730070; 3. 甘肃中医药大学定西校区生化系, 甘肃定西 743000)
• 出版日期:2016-12-20 发布日期:2016-12-20

### Effects of elevated CO2 concentration on nutrient contents and digestive enzyme activities in two color morphs of the pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae)

LI Run-Hong1,2,3, LIU Chang-Zhong2,*

1. (1. College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, China; 2. College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China; 3. Department of Biochemistry, Gansu University of Chinese Medicine, Dingxi, Gansu 743000, China)
• Online:2016-12-20 Published:2016-12-20

Abstract: 【Aim】 To clarify the physiological mechanisms of the red and green color morphs of the pea aphid, Acyrthosiphon pisum (Harris), in response to elevated CO2 concentration. 【Methods】 Two elevated levels of CO2 concentration (moderate: 550±27 μL/L; doubling: 750±37 μL/L) were manipulated in incubators with ambient CO2 (375±18 μL/L) as the control. Vicia faba seedlings were planted in the above incubators, nymphs of the pea aphid within 6 h were introduced later, and molting and the survival in various developmental stages were recorded until the adult. Nutrient contents and digestive enzyme activities of two color (red and green) morphs of the pea aphid in three successive generations and nutrient contents in V. faba leaves were assayed. 【Results】 Elevated CO2 concentration significantly enhanced the contents of soluble protein, soluble carbohydrates, free amino acids and starch in V. faba leaves. Under the moderate CO2 concentration (550±27 μL/L) condition, the nutrient contents in F0 generation of the two color morphs of the pea aphid had no significant difference as compared with the control, while the contents of protein, total amino acids, soluble carbohydrates and glycogen in F2 generation all increased and the contents of total lipid and free fatty acids decreased significantly, and moreover the content of soluble carbohydrates in F2 generation of green pea aphid was significantly higher than that in red pea aphid. Under the doubling CO2 concentration (750±37 μL/L) condition, the contents of protein, total amino acids, soluble carbohydrates and glycogen in three successive generations of the two color morphs all increased significantly and the contents of total lipid and free fatty acids decreased significantly as compared with the control, and moreover the contents of protein, total amino acids, and glycogen in the F1 and F2 generations of green pea aphid were significantly higher than those in red pea aphid. Under the elevated CO2 concentration (550±27 and 750±37 μL/L) conditions, the digestive enzyme activities in F0 generation of two color morphs of the pea aphid had no significant difference as compared with the control, while the pepsin and trypsin activities in F2 generation decreased significantly and those of lipase and amylase increased significantly, and moreover the digestive enzyme activities had no significant difference between the two color morphs.【Conclusion】 Under the elevated CO2 concentration, the change in nutrient composition in broad bean leaf causes the change in nutrient composition and digestive enzyme activities in pea aphids feeding the disposed V. faba leaves. Additionally, the changes in contents of protein, total amino acids, soluble carbohydrates and glycogen in the green morph of the pea aphid are more significant than those in the red morph. These results provide some references for controlling the pea aphid, the main pest in leguminous plants, under the elevated atmospheric CO2 concentration condition in the future.