›› 2017, Vol. 60 ›› Issue (7): 790-798.doi: 10.16380/j.kcxb.2017.07.007

• 研究论文 • 上一篇    下一篇

吡蚜酮对白背飞虱的发育、繁殖和药剂敏感性的亚致死效应(英文)

龙贵云1, 刘磊磊1, 杨洪1, 2, 王召1, 3, 金道超1,*, 周操1   

  1. (1. 贵州大学昆虫研究所/贵州山地农业病虫害重点实验室, 贵阳 550025; 2. 贵州大学烟草学院, 贵阳 550025; 3. 凯里学院环境与生命科学学院, 贵州凯里 556011)
  • 出版日期:2017-07-20 发布日期:2017-07-20

Sublethal effects of pymetrozine on the development, reproduction and insecticidal susceptibility of Sogatella furcifera (Hemiptera: Delphacidae) (In English)

LONG Gui-Yun1, LIU Lei-Lei1, YANG Hong1, 2, WANG Zhao1, 3, JIN Dao-Chao1, *, ZHOU Cao1   

  1. (1. Institute of Entomology, Guizhou University/Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Guiyang 550025, China; 2. College of Tobacco Science of Guizhou University, Guiyang 550025, China; 3. College of Environment and Life Sciences, Kaili University, Kaili, Guizhou 556011, China)
  • Online:2017-07-20 Published:2017-07-20

摘要: 【目的】白背飞虱Sogatella furcifera (Horváth)是我国重要的水稻害虫。本研究旨在确定亚致死浓度吡蚜酮对白背飞虱发育和繁殖及亚致死浓度处理后白背飞虱对其他药剂的敏感性变化的影响。【方法】用生命表法研究了亚致死浓度吡蚜酮处理对白背飞虱F0及F1代发育历期、存活率、羽化率和寿命的亚致死效应;采用稻茎浸渍法,测定了亚致死浓度吡蚜酮处理后白背飞虱对其他药剂的敏感性。【结果】经LC10和LC25吡蚜酮处理后,白背飞虱F0代3-5龄若虫历期比对照 (8.25 d)分别延长了1.98 d和4.25 d, 寿命分别比对照(14.54 d)缩短了0.49和1.73 d, 3-5龄若虫存活率(分别为85.00% 和 68.50%)和羽化率(分别为75.89%和67.78%)比对照(分别为92.00%和85.90%)下降,其中LC25处理组与对照比差异显著。LC10和LC25吡蚜酮处理的白背飞虱F1代羽化率(分别为76.97%和68.94%)、交尾率(分别为77.79%和66.44%)和卵孵化率(分别为73.19%和68.67%)比对照(分别为88.22%, 86.67%和87.26%)显著降低,但只有LC25处理组的F1代发育历期与对照组有显著差异;LC10和LC25吡蚜酮处理后的F1代单雌产卵量比对照(147.80粒/雌)显著下降(分别为112.36和88.34粒/雌),种群相对适合度比对照(1.00)也明显下降(分别为0.41和0.20)。经吡蚜酮LC10和LC25处理后,测定白背飞虱对噻虫嗪, 毒死蜱和噻嗪酮的敏感性结果显示,这3种杀虫剂的LC50 在LC10处理组中分别为2.16, 40.87和 3.12 mg/L,在LC25处理组中分别为4.93, 17.96和 8.39 mg/L。噻虫嗪、毒死蜱、噻嗪酮的相对毒力指数(RTI)结果显示,LC10和LC25处理后的白背飞虱对这3种杀虫剂的敏感性均降低。【结论】吡蚜酮处理后,白背飞虱对其他药剂的敏感性均降低。吡蚜酮亚致死浓度处理能降低白背飞虱的繁殖力,抑制种群增长,亚致死浓度处理后的白背飞虱种群对噻虫嗪、毒死蜱和噻嗪酮的敏感性均降低。

关键词: 白背飞虱, 吡蚜酮, 亚致死效应, 适合度, 种群趋势指数, 药剂敏感性

Abstract: 【Aim】 The white-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) is an important insect pest of rice. The present study aims to determine the sublethal effects of pymetrozine on the development and reproduction of this insect and its susceptibility to other insecticides. 【Methods】 The life table method was used to evaluate the sublethal effects of pymetrozine (LC10 and LC25) on developmental duration, survival rate, emergence rate and longevity of F0 and F1 generations of S. furcifera and rice stem dipping method was adopted to determine its susceptibility to other insecticides after pymetrozine (LC10 and LC25) treatment. 【Results】 The developmental duration of the F0 generations of S. furcifera treated with sublethal concentrations (LC10 and LC25) of pymetrozine for 48 h was increased by 1.98 d and 4.25 d, the longevities were shortened by 0.49 d and 1.73 d, the survival rates (85.00% and 68.50%, respectively) of the 3rd-5th instar nymphs and the emergence rate (75.89% and 67.78%, respectively) decreased as compared with the control (92.00% and 85.90%, respectively), but significant difference was just observed in generation duration between the LC25 treatment group and the control. Furthermore, the F1 generation of S. furcifera exposed to sublethal concentrations (LC10 and LC25) of pymetrozine for 48 h had decreased emergence rate (76.97% and 68.94%, respectively), copulation rates (77.79% and 66.44%, respectively), egg hatching rates (73.19% and 68.67%, respectively), fecundity (112.36 and 88.34 eggs laid per female, respectively), and relative fitness (0.41 and 0.20, respectively) as compared with the control (88.22%, 86.67%, 87.26%, 147.80 eggs laid per female and 1.00, respectively). The susceptibility of pymetrozine-treated S. furcifera to thiamethoxam, chlorpyrifos, and buprofezin showed that the LC50 values of the three chemicals were 2.16, 40.87 and 3.12 mg/L in LC10 treatment group, and 4.93, 17.96 and 8.39 mg/L in LC25 treatment group, respectively. The relative toxicity index (RTI) of thiamethoxam, chlorpyrifos and buprofezin to S. furcifera indicated that the susceptibility of pymetrozinetreated S. furcifera to these three insecticides was reduced. 【Conclusion】 Pymetrozine at sublethal concentrations can reduce the fecundity and population growth of S. furcifera and make it less susceptible to thiamethoxam, chlorpyrifos and buprofezin.

Key words: Sogatella furcifera; pymetrozine, sublethal effect, fitness, population trend index, insecticide susceptibility