›› 2018, Vol. 61 ›› Issue (2): 149-155.doi: 10.16380/j.kcxb.2018.02.001

• 研究论文 •    下一篇

异位表达Dichaete对果蝇足部发育的影响及其作用机制

董玮1, 孔悦1, 2, 马恩波1, 张建珍1, 张敏1, 张徐波1,*   

  1. (1. 山西大学应用生物学研究所, 太原 030006; 2. 山西大学生命科学学院, 太原 030006)
  • 出版日期:2018-02-20 发布日期:2018-02-20

Effects of ectopic expression of Dichaete on leg development of Drosophila and its underlying mechanism


 DONG Wei1, KONG Yue1, 2, MA En-Bo1, ZHANG Jian-Zhen1, ZHANG Min1, ZHANG Xu-Bo1, *   

  1. (1. Institute of Applied Biology, Shanxi University, Taiyuan 030006, China; 2. College of Life Sciences, Shanxi University, Taiyuan 030006, China )
  • Online:2018-02-20 Published:2018-02-20

摘要:  【目的】果蝇Dichaete基因是Sox家族B亚家族基因,其表达贯穿整个胚胎发育阶段,在个体发育过程中发挥重要作用。Dichaete在野生型果蝇幼虫足芽上也有微弱表达,然而其在足发育过程中的作用少有报道。本研究旨在研究异位表达Dichaete对果蝇足部形态构成的影响,探索相关的作用机制。【方法】黑腹果蝇Drosophila melanogaster en-Gal4品系雄性成虫与UAS-Dichaete转基因品系处女蝇杂交,驱动Dichaete异位表达,解剖子一代成虫足,在显微镜下观察异位表达的Dichaete对成虫足形态结构的影响;同时采用免疫组化技术检测异位表达Dichaete对果蝇足芽细胞凋亡和Distal-less (Dll) 表达的影响。【结果】异位表达Dichaete导致黑腹果蝇成虫足末端结构缺陷,引起3龄幼虫足芽细胞凋亡增加,上调了Distal-less基因的表达,但对Wg和Dpp信号均无影响;抑制细胞凋亡也无法拯救该缺陷。【结论】异位表达Dichaete导致黑腹果蝇成虫足末端结构缺陷,Dichaete可能通过上调Distal-less基因影响足部发育。

关键词: 黑腹果蝇, Dichaete; 异位表达, 足, 细胞凋亡, Distal-less

Abstract: 【Aim】 The Drosophila Dichaete gene is a gene of Sox B subfamily, which is expressed throughout embryogenesis and plays an important role in early development. Dichaete also exists in the leg disc of larvae, but little is known about its function in legs. The aim of this study is to investigate the effect of ectopic expression of Dichaete on leg development of Drosophila and to explore the underlying mechanism. 【Methods】en-Gal4 and UAS-Dichaete of Drosophila melanogaster were crossed, and Dichaete was ectopicly expressed under the control of en-Gal4 in the progeny. Legs of the progeny were cut off and their structure was observed using binocular microscope. Immunohistochemistry was used to detect cell apoptosis and Distal-less (Dll) expression in leg discs. 【Results】 The ectopic expression of Dichaete resulted in distal leg defects of adults, enhanced apoptosis in the 3rd instar larvae, and increased expression of distal-less in D. melanogaster, but had no effect on Wg and Dpp signaling activities. Inhibition of apoptosis could not rescue the leg defects. 【Conclusion】 Ectopic expression of Dichaete interferes with maintenance of distal-less gene, leading to structural defects of adult legof D. melanogaster.

Key words: Drosophila melanogaster, Dichaete, ectopic expression, leg, apoptosis, Distal-less