›› 2018, Vol. 61 ›› Issue (2): 246-254.doi: 10.16380/j.kcxb.2018.02.012

• 研究论文 • 上一篇    下一篇


江珍, 秦道正*   

  1. (西北农林科技大学植保资源与病虫害治理教育部重点实验室, 昆虫博物馆, 陕西杨凌 712100)
  • 出版日期:2018-02-20 发布日期:2018-02-20

Sperm ultrastructure of Euricania clara Kato (Hemiptera: Fulgoroidea: Ricaniidae)

JIANG Zhen, QIN Dao-Zheng*   

  1.  (Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Entomological Museum, Northwest A&F University, Yangling, Shaanxi 712100, China)
  • Online:2018-02-20 Published:2018-02-20

摘要: 摘要: 【目的】昆虫精子的超微结构在昆虫种类鉴定和亲缘关系探讨方面具有重要意义。研究蜡蝉总科(Fulgoroidea)昆虫的精子超微结构,可以为目前仍存较大争议的该类群发育关系分析提供更多证据。【方法】采用超薄切片法并结合光学电子显微镜和透射电子显微镜,观察透明疏广蜡蝉Euricania clara Kato精子形态和超微结构。【结果】透明疏广蜡蝉的成熟精子聚集成束,单根精子无多态性,由头部、颈区和长鞭毛组成。头部包括双层顶体复合体和精子细胞核;颈区可见中心粒和中心粒侧体;长鞭毛主要由一对D形的线粒体衍生物、一对鱼钩状副体和典型的9+9+2微管型轴丝结构组成。【结论】透明疏广蜡蝉的鱼钩状副体与已报道的其他蜡蝉类群的该结构大体一致,但与头喙亚目其他类群的副体结构有显著差异;此外,透明疏广蜡蝉精子线粒体衍生物的数量、大小及横切形状与组成与其他昆虫类群有较大差异,而在头喙亚目内表现出一定的一致性,但也存在明显差异。本研究可以为蜡蝉总科昆虫系统发育分析提供科学资料。

关键词: 头喙亚目, 广蜡蝉科, 透明疏广蜡蝉, 精子, 超微结构, 线粒体衍生物, 副体

Abstract: Abstract: 【Aim】 Sperm ultrastructure has great importance for species recognition and phylogenetics of insects. Studying sperm ultrastructure in the Fulgoroidea can provide more evidence towards phylogenetic analysis which, so far, remains controversial. 【Methods】Both the spermatozoon morphology and the ultrastructure of Euricania clara Kato were observed by ultramicrotomy in combination with light and transmission electron microscopy.【Results】 Mature spermatozoa of E. clara form a sperm bundle called spermatodesm, and each individual spermatozoon has no polymorphism and consists of a head, neck and long flagellum. The head includes the apical bilayered acrosome and nucleus. The neck region is comprised of a centriole and centriolar adjunct. The long flagellum mainly consists of a pair of symmetrical D-shaped mitochondrial derivatives, a pair of fishhook-shaped accessory bodies, and axoneme with the typical 9+9+2 microtubule pattern. 【Conclusion】The characteristics of fish-hook shaped accessory bodies of E. clara are generally consistent with those of other fulgoroids known to date, but are significantly different from other auchenorrhynchans. Furthermore, the number, size, and cross-section morphology and composition of spermatozoic mitochondrial derivatives of E. clara differ significantly from those of other insects. Although there is some consistency within the suborder Auchenorrhyncha, there are obvious differences. This study provides scientific data for phylogenetic analysis of the Fulgoroidea.

Key words: Auchenorrhyncha; Ricaniidae, Euricania clara, sperm, ultrastructure, mitochondrial derivative, accessory body