普通大蓟马聚集信息素的分离和鉴定

doi:10.16380/j.kcxb.2019.09.002

昆虫学报 ›› 2019, Vol. 62 ›› Issue (9): 1017-1027.doi: 10.16380/j.kcxb.2019.09.002

普通大蓟马聚集信息素的分离和鉴定

李晓维^1,#, 罗雪君^2,#, 王丽坤¹, 章金明¹, 张治军¹, 黄俊¹, 吴建辉^2,*, 吕要斌^1,*

(1. 浙江省农业科学院植物保护与微生物研究所, 省部共建农产品质量安全国家重点实验室(筹), 杭州 310021; 2. 广东省害虫生物防治工程技术研究中心, 华南农业大学农学院, 广州 510642)

出版日期:2019-09-20 发布日期:2019-09-03

Isolation and identification of the aggregation pheromone in Megalurothrips usitatus (Thysanoptera: Thripidae)

LI Xiao-Wei^1, ^#, LUO Xue-Jun^2,#, WANG Li-Kun¹, ZHANG Jin-Ming¹, ZHANG Zhi-Jun¹, HUANG Jun¹, WU Jian-Hui^2,*, LU Yao-Bin^1,*

(1. State Key Laboratory for Quality and Safety of Agroproducts, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2. Engineering Technology Research Center of Agricultural Pest Biocontrol in Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, China)

Online:2019-09-20 Published:2019-09-03

摘要/Abstract

摘要：

【目的】普通大蓟马Megalurothrips usitatus是我国华南地区重要的豆科害虫，给我国豇豆种植产业带来巨大损失。开发利用基于聚集信息素的引诱剂是普通大蓟马的绿色防控措施之一，对于普通大蓟马生物防治具有重要意义。【方法】采用Y型嗅觉仪测定普通大蓟马雌雄成虫对其雌虫和雄虫气味源的嗅觉反应；用顶空固相微萃取法(head space solid-phase microextraction, HS-SPME)和溶液浸提法提取普通大蓟马雌雄成虫挥发物；应用气质联用仪(gas chromatography-mass spectrometry, GC-MS)对收集的气体挥发物和正己烷提取物进行活性成分分离鉴定；应用Y型嗅觉仪测定人工合成聚集信息素组分对雌雄成虫的引诱效果。【结果】行为学试验结果表明，普通大蓟马雄成虫气味源对其雌雄成虫均具有显著的引诱作用，而雌成虫气味源则没有。顶空固相微萃取法和溶液浸提法均可提取雄成虫挥发物主要组分，但顶空固相微萃取法获得的TIC图基线平稳，杂峰少，提取效果优于溶剂浸提法。GC-MS分析结果表明，普通大蓟马雄成虫挥发物中主要含有 1种化合物，为反式,反式-金合欢醇乙酸酯［(E,E)-farnesyl acetate］，该化合物在雌成虫挥发物中不存在。室内行为学实验结果表明，特定剂量的人工合成的(E,E)-farnesyl acetate对普通大蓟马雌雄成虫均具有显著的引诱作用。【结论】本研究证明了普通大蓟马雄成虫可以释放聚集信息素，并鉴定了其主要组分(E,E)-farnesyl acetate，为应用聚集信息素开展普通大蓟马的监测和诱杀提供了基础。

关键词: 普通大蓟马, 聚集信息素, 嗅觉反应, 化合物鉴定, 反式,反式-金合欢醇乙酸酯

Abstract: 【Aim】 Megalurothrips usitatus is an important leguminous pest in South China and has caused enormous losses to cowpea planting industry. Development and application of lures based on aggregation pheromone is one of the environmentally friendly control methods for M. usitatus, which would play a key role in bio-control of this pest. 【Methods】 Behavioral responses of female and male adults of M. usitatus to headspace volatiles collected from female and male conspecifics were tested in a Y-tube olfactometer. Headspace volatiles released by female and male adults were collected using headspace solid-phase microextraction (HS-SPME) and hexane extraction. The active compound in the volatiles and hexane extracts was identified by gas chromatography-mass spectrometry (GC-MS). The behavioral responses of female and male adults to the synthetic aggregation pheromone component were tested in a Y-tube olfactometer. 【Results】 Behavioral results showed that both male and female adults of M. usitatus were attracted to the odors released by conspecific males but not those released by females. The volatiles from female and male adults could be extracted by both SPME and hexane extraction, but the extraction with SPME was more effective and suitable. GC-MS analysis showed that there was one major compound ［(E,E)-farnesyl acetate］ in the headspace volatiles from male adults, and this compound was absent from the headspace volatiles from female adults. Olfactometer results showed that both male and female adults showed significant preference to synthetic (E,E)-farnesyl acetate at certain doses. 【Conclusion】 This study confirmed the presence of aggregation pheromone in M. usitatus male adults and identified its active compound as (E,E)-farnesyl acetate, providing a basis for population monitoring and control of M. usitatus using aggregation pheromone.

Key words: Megalurothrips usitatus, aggregation pheromone, olfactory response, compound identification, (E,E)-farnesyl acetate

李晓维, 罗雪君, 王丽坤, 章金明, 张治军, 黄俊, 吴建辉, 吕要斌. 普通大蓟马聚集信息素的分离和鉴定[J]. 昆虫学报, 2019, 62(9): 1017-1027.

LI Xiao-Wei, LUO Xue-Jun, WANG Li-Kun, ZHANG Jin-Ming, ZHANG Zhi-Jun, HUANG Jun, WU Jian-Hui, LU Yao-Bin. Isolation and identification of the aggregation pheromone in Megalurothrips usitatus (Thysanoptera: Thripidae)[J]. Acta Entomologica Sinica, 2019, 62(9): 1017-1027.

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普通大蓟马聚集信息素的分离和鉴定

Isolation and identification of the aggregation pheromone in Megalurothrips usitatus (Thysanoptera: Thripidae)

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