灰茶尺蠖成虫复眼的超微结构及其明暗适应中的变化

doi:10.16380/j.kcxb.2022.10.004

昆虫学报 ›› 2022, Vol. 65 ›› Issue (10): 1277-1286.doi: 10.16380/j.kcxb.2022.10.004

灰茶尺蠖成虫复眼的超微结构及其明暗适应中的变化

许曼飞^1,2, 李孟园², 姜岩², 孟召娜², 谭畅², 王国昌^1,*, 边磊^2,*

(1. 河南科技学院资源与环境学院, 河南新乡 453003; 2. 中国农业科学院茶叶研究所, 农业部茶树生物学与资源利用重点实验室, 杭州 310008)

出版日期:2022-10-20 发布日期:2022-11-27

Ultrastructure of the compound eye of adult Ectropis grisescens (Lepidoptera: Geometridae) and its changes upon light/dark adaptation

XU Man-Fei^1,2, LI Meng-Yuan², JIANG Yan², MENG Zhao-Na², TAN Chang², WANG Guo-Chang^1,*, BIAN Lei^2,*

(1. School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China; 2. Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China)

Online:2022-10-20 Published:2022-11-27

摘要/Abstract

摘要： 【目的】明确灰茶尺蠖Ectropis grisescens成虫复眼的超微结构及其明暗适应中的变化，探究其调光机制。【方法】采用超景深显微镜测定了灰茶尺蠖成虫复眼的小眼数量、间角、直径和曲率半径等外部参数，并通过组织切片、光学显微镜和透射电子显微镜等技术观察了复眼的内部超微结构；通过光学显微镜观察了灰茶尺蠖成虫复眼在明暗环境中分别适应2 h后晶锥结构及色素颗粒的位置变化。【结果】灰茶尺蠖成虫复眼呈半球形，雌、雄虫单个复眼分别有2 502±105和3 123±78个小眼。小眼自远端至近端由角膜、晶锥、透明区构成的屈光层和由15个视网膜细胞构成的感光层组成。2个初级色素细胞包裹着晶锥，自角膜近端延伸至视网膜细胞核区的远端；每个小眼外围由6个次级色素细胞围绕，自角膜近端延伸至基膜；在透明区内14个视网膜细胞聚集成束(非感杆束)，远端与晶锥束末端连接，在感光层内形成闭合型感杆束，延伸至第15个视网膜细胞(基部视网膜细胞)。在明暗适应时，灰茶尺蠖复眼的晶锥细胞间出现开闭，色素颗粒进行纵向位移，以适应外界的光强度的变化。【结论】灰茶尺蠖成虫复眼属于重叠像眼，感杆束为“14+1”模式；屏蔽色素颗粒的移动是其复眼适应外界光强度变化的重要机制。

关键词: 灰茶尺蠖, 重叠像眼, 外部形态, 超微结构, 调光

Abstract: 【Aim】 The ultrastructure of the compound eye of Ectropis grisescens adults and its changes upon light/dark adaptation were observed to explore its light tuning mechanism. 【Methods】 The external parameters including the ommatidial number, interommatidial angle of ommatidium, ommatidial diameter and radius of curvature of ommatidium of the compound eye of E. grisescens adults were determined by using ultra depth of field microscopye, and the ultrastructure of adult compound eyes was observed by using histological sections, optical microscope and transmission electron microscope. The changes of crystalline cone structure and displacement of pigment particles in the compound eye of E. grisescens adults after adaption in light and dark environments, respectively, for 2 h were observed by using optical microscope. 【Results】 The compound eyes of E. grisescens adults are hemispherical, and each compound eye consists of about 2 502±105 ommatidia in females, and 3 123±78 ommatidia in males. Each ommatidium consists of a dioptric apparatus made up of a cornea, a crystalline cone and a clear zone, and a photosensitive layer made up of 15 retinal cells. Two primary pigment cells encase the crystalline cone and extend from the proximal part of the cornea to the distal part of the retinula cell nuclear zone. The periphery of ommatidium is surrounded by six secondary pigment cells, which extend from the proximal part of the cornea to the basement membrane. In the clear zone, 14 retinal cells aggregate into a bundle (non-rhabdom), the distal part of which is connected with the end of the crystalline tract, forming a closed rhabdom in the photosensitive layer and extending to the 15th retinula cell (basal retinula cell). During light/dark adaptation, the compound eyes adjust the light input mainly by the longitudinal displacement of pigment particles and the opening and closing of crystalline cone to adapt to the change of light intensity. 【Conclusion】 The compound eye of E. grisescens adults belongs to the refracting superposition eye, and the rhabdom is the 14+1 pattern. The movement of pigment particles is an important mechanism for the compound eyes of E. grisescens adults to adapt to the change of light intensity.

Key words: Ectropis grisescens, superposition eye, external morphology, ultrastructure, light tuning

许曼飞, 李孟园, 姜岩, 孟召娜, 谭畅, 王国昌, 边磊. 灰茶尺蠖成虫复眼的超微结构及其明暗适应中的变化[J]. 昆虫学报, 2022, 65(10): 1277-1286.

XU Man-Fei, LI Meng-Yuan, JIANG Yan, MENG Zhao-Na, TAN Chang, WANG Guo-Chang, BIAN Lei. Ultrastructure of the compound eye of adult Ectropis grisescens (Lepidoptera: Geometridae) and its changes upon light/dark adaptation[J]. Acta Entomologica Sinica, 2022, 65(10): 1277-1286.

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灰茶尺蠖成虫复眼的超微结构及其明暗适应中的变化

Ultrastructure of the compound eye of adult Ectropis grisescens (Lepidoptera: Geometridae) and its changes upon light/dark adaptation

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