中华蜜蜂嗅觉受体AcerOrco的表达及定位分析

doi:10.16380/j.kcxb.2016.02.008

Abstract

Abstract: 【Aim】 By the expression and localization analysis of olfactory receptor AcerOrco in the Chinese honeybee, Apis cerana cerana, we researched the expression characteristics of AcerOrco, in order to provide a theoretical basis for further exploring its function. 【Methods】 Real-time PCR and Western blot technology were used respectively to determine the expression profiles of AcerOrco mRNA and protein in five tissues (antennae, head, thorax, abdomen and legs)of nurse and forager bees. By immunohistochemical technique, cellular localization of the olfactory receptor in antenna was analyzed.【Results】 The expression profiling showed that AcerOrco transcripts were detected in all the tested tissues of A. cerana cerana, and the most transcripts were found in antenna. The expression of this gene in different tissues of forager was generally higher than that in nurse. AcerOrco protein were also detected in all the tested tissues, and mainly expressed in antenna and head of nurse and forager. The location result showed that in nurse AcerOrco was detected in sensilla trihodea, but had no obvious expression in sensilla placodea. In forager AcerOrco was mainly expressed in sensilla placodea and sensilla trihodea, with higher expression level in sensilla trihodea. 【Conclusion】 The expression profiles of AcerOrco at mRNA and protein levels were obtained, and the encoded protein was successfully localized in sensilla placodea and sensilla trihodea of antenna of worker bee.

Key words: Apis cerana cerana, olfactory receptor, mRNA expression, protein expression, protein localization

ZHANG Zhong-Yin, YANG Shan-Shan, MENG Jiao, ZHAO Hui-Ting, JIANG Yu-Suo. Expression and localization analysis of olfactory receptor AcerOrco in the Chinese honeybee, Apis cerana cerana (Hymenoptera: Apidae)[J].Acta Entomologica Sinica, 2016, 59(2): 185-191.

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Expression and localization analysis of olfactory receptor AcerOrco in the Chinese honeybee, Apis cerana cerana (Hymenoptera: Apidae)

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