Acta Entomologica Sinica ›› 2017, Vol. 60 ›› Issue (3): 254-263.doi: 10.16380/j.kcxb.2017.03.003

• RESEARCH PAPERS • Previous Articles     Next Articles

Identification and characterization of peroxiredoxin 2 involved in the antioxidative response induced by bacterial infection in the pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae) (In English

ZHANG Yong-Dong1, LI JunLan1, CHEN Jin-Hong1, MA Li1, CHEN Kang-Kang1, LÜ Zhi-Qiang1,2,*   

  1. (1. Department of Entomology, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Northwest A&F University, Yangling, Shaanxi 712100, China)
  • Online:2017-03-20 Published:2017-03-20

Abstract: 【Aim】 The aim of this study is to reveal the function of peroxiredoxin 2 (ApPrx2) in the pea aphid, Acyrthosiphon pisum during bacterial infection. 【Methods】 The open reading frame of ApPrx2 was cloned and heterologously expressed in Escherichia coli. The antioxidative function of the purified recombinant ApPrx2 was characterized. The H2O2 concentration and ApPrx2 transcription level were detected in pea aphids after bacterial infections of Pseudomonas aeruginosa and Staphylococcus aureus. RNA interference was used to knockdown the expression of ApPrx2. H2O2 concentration, bacterial load and the survival rate of aphids were evaluated after the knockdown of ApPrx2. 【Results】 Sequence alignment showed that ApPrx2 is highly similar to 2-Cys Prxs from other insect species. In vitro assays demonstrated that the recombinant ApPrx2 protein degraded H2O2, and ApPrx2 expression rendered E. coli cells resistant to H2O2. Infections of the bacteria P. aeruginosa and S. aureus increased the H2O2 concentration and ApPrx2 expression in the pea aphids. The knockdown of ApPrx2 expression increased the H2O2 concentration, reduced the bacteria load and increased the mortality rate of aphids infected with S. aureus. 【Conclusion】 ApPrx2 functions as an antioxidative protein protecting pea aphids from stress induced by infection with bacteria, particularly S. aureus.

Key words: Acyrthosiphon pisum, peroxiredoxin, reactive oxygen species (ROS), antioxidative response, bacterial infection