Abstract: The 3′terminal of the actin genes in the brown planthopper, Nilaparvata lugens (Stal) was amplified, cloned and sequenced using the anchored 3′RACE method. Through homologous comparison of these sequences in untranslated regions, we found that BPH-A and BPH-D were different from other sequences, and that BPH-B, BPH-C and BPH-E had the same sequence except for different 3′ terminal sites. The amino acid sequence of the 3′ terminal of the actin genes in N. lugens is highly conservative. According to structural characteristics of the actin genes, we conclude that BPH-A and BPH-D are muscle specific while BPH-B, BPH-C and BPH-E are cytoplasm specific.

Key words: Nilaparvata lugens, actin, 3′ RACE, gene expression