›› 2004, Vol. 47 ›› Issue (3): 412-416.

• RESEARCH PAPERS • Previous Articles    

Cloning and analysis of two Cry1Ac receptor-like aminopeptidase N cDNA fragments from Trichoplusia ni (Hübner) cultured cell

JIANG Cai-Fu, LIU Kai-Yu, PENG Rong, PENG Jian-Xin, HONG Hua-Zhu*   

  • Online:2004-06-20 Published:2004-06-20
  • Contact: HONG Hua-Zhu

Abstract: Some aminopeptidase N (APNs) serve as Bacillus thuringiensis (Bt) Cry1Ac toxin receptor in the midguts of several lepidopteran insects. Two fragments of two different APN genes in BTI-TN-5B1-4 (a cloned cell line from Trichoplusia ni ovary) were amplified with two pairs of degenerate pri mers by RT-PCR, and the lengths of them were 188 bp and 564 bp,and named as 188(GenBank Accession: CD809324)and AS564 (GenBank Accession: CD809326)respectively. Deduced protein sequences were highly similar to that of several recept or APNs in insects, with sequence identities of 45%-47% for the shorter one and 61%-63% for the other.

Key words: Trichoplusia ni, cultured cell, aminopeptidase N, gene, clone, sequence analysis